Abstract: 　AIM: To investigate the anti hemolytic and anti membranous lipid peroxidation effects of daphnetin. METHODS: The inhibition rates of daphnetin on AQD induced hemolysis and erythrocytic membranous lipid peroxidation were determined by routine in vitro assay for detecting hemolytic toxicity and by HPLC for detecting membranous lipid peroxidation products. RESULTS: Daphnetin at a range of 10-80 μmol/L inhibited dose dependently AQD induced hemolysis and lipid peroxidation by 33 0%-69 2% and 11 9%-58 2%, respectively. CONCLUSION: Daphnetin has anti hemolytic and anti erythrocytic membranous lipid peroxidation effects.

Key words: Daphnetin, anti hemolytic, anti membranous lipid peroxidation