›› 1998, Vol. 16 ›› Issue (4): 256-259.

• 论著 • Previous Articles     Next Articles

SEQUENCING AND HOMOLOGY ANALYSIS OF cDNAS ENCODING FhGST AND AsGST *

Sun Wenyu; Liu Shuxian   

  1. Institute of Parasitic Diseases **; Chinese Academy of Preventive Medicine; Shanghai 200025
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-08-28 Published:1998-08-28

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Abstract:

AIM: To study the homologies between FhGST, AsGST and Sj26GST. METHODS: Total RNAs
were extracted from Fasciola hepatica(Fh) and Ascaris suum(As) by guanidine hydrochloride/CsCl
ultra centrifugation. Two pairs of primers were designed according to FhGST and AsGST sequences.
RT PCR was carried out using total RNA as template. PCR products were directly sequenced. Then
the homology analysis was made using DNASIS software on nucleotide level. RESULTS: A FhGST403 bp
fragment and a AsGST411 bp fragment were obtained and they both located in the open reading frame. CONCLUSION: On nucleotide level, the homologies are 59% and 54% between the two cloned fragments and Schistosoma japonicum 26 kDa GST (Sj26GST) respectively

Key words: FhGST, AsGST, RT PCR, homology