›› 1998, Vol. 16 ›› Issue (3): 181-184.

• 论著 • Previous Articles     Next Articles

ANALYSIS OF MOLECULAR KARYOTYPE OF LEISHMANIA ISOLATES FROM HILLY FOCI AND PLAIN FOCI OF CHINA

Lu Fangli1; Hu Xiaosu1; Tang Haimei2; Zeng Fanya2; Zhang Yizheng2; Dai Baomin3   

  1. 1 Department of Parasitology, West China University of Medical Sciences, Chengdu 610041 2 Department of Biotechnology, Sichuan Union University, Cheng du 6100643 Department of Pathologic Physiology , West China University of Medical Sciences, Cheng du 610041
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-06-30 Published:1998-06-30

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Abstract: AIM: To analyse the molecular karyotypes of Leishmania isolates from
hilly and plain foci of China. METHODS: Pulsed field gel electrophoresis(PFGE) was
performed in 1% agarose at 6 V/cm in 0.5×TBE at 14℃ with a pulse time of 60 s for
15 h and a pulse time 90 s for 9 h. RESULTS: Each isolate was resolved into 15
different EtBr staining bands ranging in size from 200 to 2 200 kb, in which the
karyotypes of 6 isolates from hilly foci were similar(including karyoptypes of
canine isolates similar with those of human isolates), and they were partly similar with those of L. infantum. The karyo types of the two isolates from plain foci were similar, but the isolates from the two topographically different foci were different from each other. CONCLUSION: Homology exists between L. d. isolates from hilly foci and plain foci respectively, and exists in part between L. d. isolates from hilly foci and L. infantum ; while heterogeneity exist s between L. d. isolates from hilly foci and plain foci. The reservoir host, domestic dog, plays an important role as the infection source of leishmaniasis in the hilly foci of China.

Key words: Leishmania, PFGE, hilly foci, plain foci, homology, heterogeneity