CLONING AND SEQUENCING OF THE GENE ENCODING TPI ANTIGEN FROM CHINESE STRAIN OF SCHISTOSOMA JAPONICUM

Abstract

Abstract: AIM:Cloning and sequencing the gene encoding TPI antigen from Chinese strain of S. japonicum. METHODS:RT- PCR technique was used to gain the target gene. After digestion with restriction enzyme, the gene was respectively cloned into the vectors M13mp18, M13mp19, then sequenced by the method of dideoxy- mediated chain- termination. RESULTS:The 0.75 kb c DNA encoding Sjc TPI antigen was amplified in vitro by RT- PCR using total RNA as a template. 0.75 kb insert DNA was identified in six recombinant clones by restriction enzymemapping. The DNA sequence of SjcTPI was determined. CONCLUSION:
The process have been made succesfully in the cloning and sequencing the gene encoding SjcTPI. It will be scheduled to go ahead for vaccine trial probably using recombinant TPI antigen of S.japonicum.

Key words: Schistosoma japonicum （Chinese strain）, TPI, RT- PCR, gene clone, DNA sequenc

MiaoYingxin**;LiuShuxian. CLONING AND SEQUENCING OF THE GENE ENCODING TPI ANTIGEN FROM CHINESE STRAIN OF SCHISTOSOMA JAPONICUM[J]. , 1997, 15(6): 349-352.

[1]	YU Ming-chuan, YANG Zhong-wei, WANG Hua-ran, SHI Dan-yang, ZHOU Shu-qing, YIN Jing, SUN Shu-min. Establishment of a Giardia lamblia detection method based on the LAMP microfluidic chip [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(3): 402-405.
[2]	LI Xin-yan1,2, ZHANG Zheng1, LIU Dong-li1*, WANG Feng-ping3, WANG An-li1, WANG Tian-hai3, SHI Yi1, GUAN Rong-hui1, LI Wen-juan1. Identification of Leishmania in Shaanxi Province based on repeated DNA sequence [J]. , 2018, 36(3): 21-271-274.
[3]	YUAN Fei1，LIU Zhuan-zhuan2，ZHANG Bo2，CAO Jian-ping3，ZHENG Kui-yang2 *，WANG De-guang1. Prokaryotic Expression and Immunoreactivity Analysis on Profilin of Toxoplasma gondii [J]. , 2015, 33(1): 4-21-24.
[4]	ZHOU Hong-chang，DAI Jia-li，FAN Cheng-cheng，ZHONG Hui-ya，SHEN Yu-qing. Cloning and Bioinformatics Analysis of PFC0460w in Plasmodium falciparum* [J]. , 2012, 30(1): 18-76-77.
[5]	YUXin-ling;HEYong-kang;XIONGTie;ZHAOYa-qin;SHIMeng-zhi;ZHOUJie;LIUZong-chuan;LUOXin-song;FUXiao;HEHong-bin;D.A.HARN;LIYue-sheng. Protective Effects of Co-Immunization with SjCTPI-Hsp70 andInterleukin-12 DNA Vaccines against Schistosoma japonicumChallenge Infection in Water Buffalo [J]. , 2006, 24(6): 7-436.
[6]	ZHAOGen-ming;WANGLi-ying;ZHAOQi;CHENXian-yi;XIAODong-lou;HENa;WEIJian-guo;JIANGQing-wu. National Surveillance of Schistosomiasis in China from 2000 to 2004 [J]. , 2006, 24(1): 2-9.
[7]	LIXiao-hong;LIUShu-xian;SONGGuang-cheng;XUYu-xin;CAOJian-ping;CHENJia-xu. Screening of cDNA Library of Schistosoma japonicum with Sera from Rabbits Vaccinated with Ultraviolet-attenuated Schistosomula [J]. , 2004, 22(2): 4-82.
[8]	CUIAi-li;CHANGZheng-shan;CHENMing-gang;DavidBlair;CHENShao-hong;ZHANGYong-nian;FENGZheng. Taxonomic Status in DNA Sequences of Five Species of Genus Paragonimus [J]. , 2003, 21(1): 9-30.
[9]	SHENJi-jia;JIANGZuo-jun;YUXin-bing;WANGXue-long;WANGWei. Cloning and Identification of an Unknown Gene Encoding 10.6 kDa Protein of Schistosoma japonicum [J]. , 2001, 19(3): 7-159.
[10]	ZHOUCheng;YAOHangping;WENTinghuan;SUNCuiru. Effect of Immunostimulatory DNA Sequence on the Production of Th1 and Th2 Cytokines Induced by Dermatophagoides farinae Allergen in vitro [J]. , 2001, 19(2): 1-67.
[11]	YUChuanxin;ZHUYinchang;YINXuren;HEWei;XUYongliang;GUANXiaohong. Screening the Mimic Antigen Epitopes of Triosephosphate Isomerase of Schistosoma japonicum Chinese Strain (Sjc Tpi) with Random Phage Peptide Library [J]. , 2001, 19(1): 4-14.
[12]	CAOJianping;LIUShuxian. Cloning and Expression of the Gene Encoding Schistosoma japonicum Tropomyosin * [J]. , 2001, 19(1): 7-25.
[13]	WANGQing-min;DAIJian-xin;ZHANGPing-wu;SUNShu-han. CLONING OF CYSTICERCUS CELLULOSAE AgB cDNA CODING REGION [J]. , 2000, 18(2): 3-75.
[14]	ZHOUShenghua;LIUShuxian;SONGGuangcheng;XUYuxin. CLONING,SEQUENCING AND EXPRESSION OF THE FULL-LENGTH GENE ENCODING PARAMYOSIN OF SCHISTOSOMA JAPONICUM IN VIVO * [J]. , 1999, 17(4): 2-199.
[15]	onclick="TPI_openwindow('/KNS0/knowledgenet.aspx?sdb=CJFD&sfield=%e%b%b%e%%%e%bd%c%e%0%&skey=ZHANG++Shuyi';'knet';'status=yes;scrollbars=yes;width=;height=0')">ZHANGShuyi;onclick="TPI_openwindow('/KNS0/knowledgenet.aspx?sdb=CJFD&sfield=%e%b%b%e%%%e%bd%c%e%0%&skey=WEI++Meixiong';'knet';'status=yes;scrollbars=yes;width=;height=0')">WEIMeixiong;onclick="TPI_openwindow('/KNS0/knowledgenet.aspx?sdb=CJFD&sfield=%e%b%b%e%%%e%bd%c%e%0%&skey=ZHAO++Huifen';'knet';'status=yes;scrollbars=yes;width=;height=0')">ZHAOHuifen;onclick="TPI_openwindow('/KNS0/knowledgenet.aspx?sdb=CJFD&sfield=%e%b%b%e%%%e%bd%c%e%0%&skey=SHI++Gongfen++Shanghai++Detecting++Center++for++Toxoplasmosis++Shanghai++Institute++of++Parasitic++Disease++Control++and++Rsesarch';'knet';'status=yes;scrollbars=yes;width=;height=0')">SHIGongfen. ESTABLISHMENT OF IMMUNOGLOBULIN M(IgM)\\|IMMUNOSORBENT AGGLUTINATION ASSAY (ISAGA) FOR DIAGNOSIS OF TOXOPLASMOSIS [J]. , 1999, 17(4): 11-227.

CLONING AND SEQUENCING OF THE GENE ENCODING TPI ANTIGEN FROM CHINESE STRAIN OF SCHISTOSOMA JAPONICUM

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments