Abstract: AIM:Cloning and sequencing the gene encoding TPI antigen from Chinese strain of S. japonicum. METHODS:RT- PCR technique was used to gain the target gene. After digestion with restriction enzyme, the gene was respectively cloned into the vectors M13mp18, M13mp19, then sequenced by the method of dideoxy- mediated chain- termination. RESULTS:The 0.75 kb c DNA encoding Sjc TPI antigen was amplified in vitro by RT- PCR using total RNA as a template. 0.75 kb insert DNA was identified in six recombinant clones by restriction enzymemapping. The DNA sequence of SjcTPI was determined. CONCLUSION:
The process have been made succesfully in the cloning and sequencing the gene encoding SjcTPI. It will be scheduled to go ahead for vaccine trial probably using recombinant TPI antigen of S.japonicum.

Key words: Schistosoma japonicum （Chinese strain）, TPI, RT- PCR, gene clone, DNA sequenc