Abstract: Serum samples were collected from normal rabbits,normal persons, Schistosoma japo nicum infected rabbits and human cases respectively,and were treated by TCA precipitation and PEG precipitation,and subsequently detected by dot ELISA.The results showed that both the supernatants of TCA treated antisera from infected human cases and rabbits and the precipitants of PEG treated antisera of the same origins showed positive reactions,while no positive reactions were found for sera from normal rabbits and normal persons.It was exhi bited that dot ELISA detected not only the circulating antigen of schistosomes but also the immunocomplex.