Abstract: The DNA of Plasmodium falciparum has been purified and fragmented with restriction endonuclease BamHI. The fragments have been incorporated in vitro into derivatives of bac-teriophage lambda EMBL4 digested with BamHI and Sal I. The recombinant mixture has been ligated and packaged in vitro. The recombinant phages have been identified in E. coli L95host cell and the libraries have been established in which most of the parasite DNA is represented. The ligation proportion of vector to insert is 3 : 1. The recombinant phages of 4×105 have been obtained. By plaque hybridization, we have been able to recover from these libraries specific clones containing repetitive DNA sequences.