›› 1990, Vol. 8 ›› Issue (4): 267-269.
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Abstract: Monoclonal antibodies were produced by the fusion of splenic lymphocytes from BALB/c mice immunized with Schistosoma japonicum excretory / secretory antigen and the myeloma cell line SP2/0. The 1B2E7B8 McAb was proved to be specific against the gut antigen of adult worm in IFA. The McAb labelled with HRP was used in Dot-ELISA to detect schistosome circulating antigen.Schistosome circulating antigen was detected in 152 out of 188 proven cases of schis-tosomiasis, accounting for a positive rate of 80.9%. The positive rates for circulating antigen in cases with 1-24, 25-99 and ≥100 EPG were 76.8%, 86.6% and 100% respectively. 10 out of 11 cases who had been checked 2 months after effective treatment became ELISA negative. No circulating antigen was detected in cases with other parasitosis nor in normal individuals. In addition, the McAb-Dot-ELISA showed good reproducibility The results indicated that McAb-Dot-ELISA might be used for diagnosis of schistosomiasis and evaluation of cure.
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https://www.jsczz.cn/EN/Y1990/V8/I4/267