Abstract: We have tried to use 1.2cm × 5cm (6cm2) adhesive cellophane tape technique for quantitative detection of Demodex. 618 mites were detected on the 24 tapes applied on various parts of the face of a healthy individual.In an investigation of 8 person-times, the numbers of mites found on various parts of the face were in the following order: upper-cheek(233), mid-cheek(153), lower-cheek (114), chin(78), forehead(59.5), nose(55), infraorbital(54.5), paraoral(49.5), nasal groove(45), mites found on other parts being negligible.Demodex brevis were found scattering singly on the tapes, while D. follicutorum were found 1-6 in number in one or several follicles. All of them were in the follicle opening, indicating that transmission was readily affected through direct or indirect contacts.The results also showed that when a large number of mites were sticked off, the intensity of infestation was decreased.