Abstract: In the studies presented here, we demonstrated the feasibility of producing large number of hybridoma cell lines secreting McAbs against S. japonicum including 16 cell lines secreting IgG McAbs (7 for IgG1, 5 for IgG2a, 1 for IgG2b, 3 for IgG3) and 13 McAbs for IgM, on the basis of successfully extracting 24-26kD and 90kD "target an tigen" proteins known as important antigens for inducing schistosome protective immunity All the above cell lines were characterized for localization of the McAbs, mediating in vitro ADCC against schistosomula, epitope recognized by the McAbs on different kinds of schistosome antigens. Studies have shown the evidences that the McAbs can be used to identify the "target antigen" on the surface of schistosome, to isolate and purify "target antigen" of S. japonicum by chromatography column bearing McAbs, as well as to immunize animals as antigens for accumulation of data in the development of vaccine against S. japonicum via anti-idiotype antibodies.