Abstract: The ovarian nurse cell's polytene chromosomes of An. minimus exist as 5 arms, the telocentric X chromosome, the submetacentric chromosome 2 and the metacentric chromosome 3. The X chromosome is easily recognized by its length and shuttle-shaped zone 6, The most important characteristics is that each autosome arm has one to three big puffs. Among the 46 zones, 7A, B and 19C in 2R which is the longest arm in the complement, 28A and 20A, B in 2L, 30A, B and 37D in 3R and 46D and 38A, B in 3L are considered as characteristic zones. It is proposed that this map might be eonsn dered as the "standard" map of the ovarian nurse cell's polytene chromosomes for An, minimus from Guangxi (Fig. 1).