IMMUNOLOGICAL HUMORAL RESPONSE IN MICE EXPERIMENTALLY INFECTED WITH ECHINOCOCCUS GRANULOSUS, PRELIMINARY RESULT OF KINETIC OBSERVATION

›› 1989, Vol. 7 ›› Issue (3): 170-173.

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IMMUNOLOGICAL HUMORAL RESPONSE IN MICE EXPERIMENTALLY INFECTED WITH ECHINOCOCCUS GRANULOSUS, PRELIMINARY RESULT OF KINETIC OBSERVATION

  

  • Received:2017-01-09 Revised:2017-01-09 Online:1989-08-31 Published:2017-01-09

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Abstract: 5 serological methods included latex agglutination test (LA), enzyme-linked immu-noelectrotransfer blot (EITB), enzyme-linked immunosorbent assay(ELISA), dot-enzyme-linked immunosorbent assay (Dot-ELISA) and immunofluorescence antibody test (IFA) were used to trace tne kinetic changes of the humoral response in NIH mice experimen-tally infected with protoscolex of Echinococcus granulosus. Results showed that Dot-ELISA and IFA were able to detect antibodies 2 wk after infection while ELISA, EITB and LA gave positive reactions 4 wk, 2 mos and 3 mos after infection respectively. Therefore Dot-ELISA and IFA were considered to be the most sensitive ones among the 5 methods used. All infected mice gave positive reactions at 8 and 12 mos but none of the normal control mice showed any reaction throughout the period of observation (Fig. 1).