Abstract: Dot-Immunobinding assay was adapted for tne indircct scrological measuremcnt of anti-Echinococcus antibodies in human hydatid discasc. The sensitivity, specificity and reproducibility were evaluated by testing 56 sera from patients with clinically diag-nosed or surgically confirmed hydatidosis, 39 sera from patients with non-hydatid disease (liver cirrhosis, hepatitis, non-parasitic lung cyst, different types of cancer, tuberculosis and cysticercosis) and 60 sera from healthy adults. The dilution of sera was 1:400 and an cxtinction value of 0.1 was considercd as positive reaction.The results indicated that the sensitivity and specificity of this test were 98.9% and 97.0% respectively. The extinction values (mean±SD) of sera from paticnts with. hydatid disease, non-hydatid disease and from healthy adults were 0.515±0.195, 0.095 ±0.045, 0.055 ± 0.045 respectively.Comparison of the quantitativc test with O-phenylenediamine as substrate which gives yellow soluble product with the qualitative test with benzidine-HCL as substrate which gives a bluish brown precipitate adhering to the nitrocellulose membrane showcd that the qualitative test is as sensitive and specific as the quantitative one, but the formcr is more simple and convenient to perform than the latter.