Abstract: A new strain of Plasmodium falciparum was isolated from Jiangsu, China and coded FCC102/JS in continuous cultivation for a period of 52 days by Trager-Jensen's Candle Jar method with gametocytes found over the whole period (Table 1). This strain differed distinctly in growth rates from FCC1/HN and FCC4/HN isolated from Hainan Island. The mean multiplication rate in 96 hrs of FCC102/JS was 3.83 ±1.13,while that of FCC1/BN and FCC4/HN was 10.03 ±0.88 and 9.85 ±0.38,respectively. The results of 48 hrs in vitro microtechnique showed that FCC102/JS was sensitive to chloroquine with a mean IC50 of 42.03(28.51-61.95) nM (Table 2). It was suggested that there may be some biological differences between FCC102/JS strain and FCC1/HN as well as between FCC102/JS and FCC4/HN strain.