Abstract: Adult Thelazia callipaeda collected from naturally infected dogs were transplanted in the conjunctival sac of laboratory rabbits and found surviving for 23 months after transplantation, Those worms maintained in physiological saline solution at 20℃, survived for 58 hours, but lost the capacity to infect experimental animals after 48 hours preservation.This parasite caused inflammatory reaction within 3 to 48 hours after transplantation in the eyes of rabbits. The severity of inflammatory response appears to be closely associated with the number of adult worms transplanted.