Abstract: The chromosomes of salivary glands of Anopheles sinensis from Zhengzhou (Henan), Shanghai and Youxi (Fujian) districts were studied in 1979-1982 to detect their subspecies classification.The main differences among the gland chromosomes of the 4th instar larvae in these three districts were on the free ends of 2R besides tiny changes on each arm.The characteristics of chromosomes in Youxi was more identical with those in Shanghai than in Zhengzhou. It is seemingly due to the geographical variation.X chromosomes of Anopheles sinensis in these three districts were basically similar one to another, but they vary in position, size and component, especially in the puff position.Compared with Anopheles sinensis from Nanjing, the variance among salivary gland chromosomes of Anopheles sinensis 4th instar larvae renders another evidence of chromosomal polymorphism in the biological world as a whole.