Surveillance and analysis of parasitic infection in food on market in Shanghai during 2015—2019

doi:10.12140/j.issn.1000-7423.2021.03.008

Abstract

Abstract:

Objective To understand the status of parasitic infection in the food on market in Shanghai, in order to provide evidence for formulating strategies for food safety. Methods To carry out the surveillance,freshwater products, seawater products, pickled products, meat products and vegetables on farm product markets and supermarkets were sampled in Shanghai during 2015—2019, based on the combination of urban and suburb regions. The parasitic infection in the food samples were examined by using pressing method and digestion for detecting metacercariae in freshwater products and pickled products; using dissection microscopy forAnisakis larvae in seawater products; using pressing method for Taenia cysticercus and Trichinella encysted larvae in meat products; using fluorescent quantitative PCR for detectingToxoplasma gondii DNA in the meat products; using the saline floating method for Fasciolopsis buski metacercaria or parasite eggs in vegetables. Results A total of 1 125 samples of fresh water products were examined during 2015—2019, of which 9 were found of parasite metacercariae, with a positive rate of 0.8%. In the 585 freshwater fish samples, the positive rate of parasite metacercariae (Clonorchis sinensis) was 1.5% (9/585). The 540 samples of freshwater shrimps and crabs were not detected of Paragonimus metacercariae. Among freshwater fishes, the Pseudorasbora parve had the highest positive rate (5/8). The positive rates of parasite metacercaria in freshwater fishes were 0.7% (1/144), 3.3% (4/121), 1.7% (2/120), 0 (0/100) and 2.0% (2/100) during 2015—2019, respectively.Anisakis larvae were found in 31.1% (184/591) of 591 samples of 35 species of seawater products. The positive rates of Anisakis larvae in seawater fishes were 36.0% (50/139), 37.1% (49/132), 29.2% (35/120), 39.0% (39/100) and 11.0% (11/100) during 2015—2019, respectively. The positive rate ofAnisakis was higher in Pneumatophorus japonicas (72.5%, 37/51), Larimichthys polyactis (70.1%, 61/87) and Trichiurus lepturus (50.0%, 52/104) than in other seawater products (19.1%, 34/178) (χ² = 33.9, 48.3, 13.2; P < 0.01). Among the 443 pickled products examined, 272 pickled bullacta exarata products were infected with Echinostoma metacercariae, showing a positive rate of 61.4%, and none was detected with Paragonimus metacercariae. Of the 253 meat product samples of pigs, cattle, sheep, chickens and ducks, none was detected with Taenia solium metacercariae,Taenia saginata metacercariae, Trichinella larva cysts, or Toxoplasma spp. The 30 samples of vegetables were not detected with Fasciolopsis buski metacercaria or other parasite eggs. Conclusion There are different degrees of parasitic infection in freshwater products, seawater products and pickled products on the market in Shanghai.

Key words: Food-borne parasites, Monitoring, Food sold in market, Shanghai City

CLC Number:

WANG Zhen-yu, WU Huan-yu, JIANG Li, MA Xiao-jiang, ZHANG Yao-guang, HE Yan-yan, ZHU Qian. Surveillance and analysis of parasitic infection in food on market in Shanghai during 2015—2019[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(3): 347-351.

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鱼类 Fish species	检查份数 No. examined	阳性份数 No. positive
麦穗鱼Pseudorasbora parve	8	5
鳑鲏鱼Cyprinidae rhodeus	7	2
鲫鱼Carassius auratus	60	2
其他淡水鱼类 Other species of freshwater fishes	510	0
合计 Total	585	9