Analysis of nad1 gene polymorphisms of Echinococcus granulosus isolates from humans in Ali region of Tibet

doi:10.12140/j.issn.1000-7423.2020.01.003

Abstract

Abstract: Objective To understand the dominant genotypes and genetic variations of Echinococcus granulosus isolates from humans in Ali region, in order to provide support for tracing the origin of E. granulosus and formulating strategies for prevention and control of echinococcosis in Ali region. Methods Specimens resected from hydatidosis patients in 2017 in a hospital in Ali area were used for DNA extraction. Mitochondrial NADH dehydrogenase 1 (nad1) gene was amplified by PCR. The PCR products were sequenced and analyzed by BLAST, ClustalX 1.83 and MEGA 7.0 softwares for homology and phylogenetic analyses. The nad1 gene sequences of E. granulosus in China was downloaded and the haplotypes were analyzed by DnaSP6. The haplotype network map of nad1 gene of E. granulosus in China was drawn by the NetWork software. Results A total of 80 specimens of hydatidosis were collected, and 38 resulted in a specific band around 550 bp after PCR amplification. Sequencing analysis revealed that E. granulosus in 4 cases had the G6 genotype, which was 99.81% homogeneous with the sequence of Mongolian origin (MH300971.1). The remaining 34 cases revealed G1 genotype of E. granulosus, which had a C-to-G mutation at position 535 as compared to the standard sequence (AF297617.1) and was 100% homogeneous with that of the Algerian origin (MG672293.1). Analysis of the 38 samples with MEGA 7.0 software showed that the bases T, C, A and G constituted 44.3%-46.5%, 7.7%-9.1%, 19.8%-21.5% and 25.7%-26.0%, respectively. The reported E. granulosus nad1 gene in China had 9 haplotypes, with a haplotype diversity of 0.47 and nucleotide diversity of 0.19. The haplotype network map showed that H4 was the main haplotype for nad1 gene in China. Conclusion G1 and G6 are the genotypes of E. granulosus nad1 gene in Ali region. The data analysis indicates that H4 is the major haplotype for E. granulosus nad1 gene in China.

Key words: Echinococcus granulosus, Genetic polymorphism, nad1 gene, Haplotype

CLC Number:

R383.33

WEI Yu-huan, LIU Hua, LI Wu-jun, ZHAO Hai, HU Yuan, CAO Jian-ping. Analysis of nad1 gene polymorphisms of Echinococcus granulosus isolates from humans in Ali region of Tibet[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(1): 17-22.

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