关键词: 细粒棘球蚴, 实验感染鼠, 体液免疫, Dot-ELISA, 寄生虫病研究所, 中国预防医学科学院, 鼠血清, 阳性反应, 棘球坳, 动态观察

Abstract: 5 serological methods included latex agglutination test (LA), enzyme-linked immu-noelectrotransfer blot (EITB), enzyme-linked immunosorbent assay(ELISA), dot-enzyme-linked immunosorbent assay (Dot-ELISA) and immunofluorescence antibody test (IFA) were used to trace tne kinetic changes of the humoral response in NIH mice experimen-tally infected with protoscolex of Echinococcus granulosus. Results showed that Dot-ELISA and IFA were able to detect antibodies 2 wk after infection while ELISA, EITB and LA gave positive reactions 4 wk, 2 mos and 3 mos after infection respectively. Therefore Dot-ELISA and IFA were considered to be the most sensitive ones among the 5 methods used. All infected mice gave positive reactions at 8 and 12 mos but none of the normal control mice showed any reaction throughout the period of observation (Fig. 1).