福建省建宁县溪蟹并殖吸虫囊蚴感染情况及基因分型

doi:10.12140/j.issn.1000-7423.2025.03.020

摘要/Abstract

摘要：

了解福建省建宁县溪蟹并殖吸虫囊蚴感染情况及其基因分型。2022年7—12月在福建省建宁县楚溪河水系邻近居民区的山涧水沟采集溪蟹，采用双筛法收集并殖吸虫囊蚴并进行形态学鉴定。提取囊蚴DNA，PCR扩增核糖体内转录间隔区2（ITS2）序列，测序后将所获的基因序列提交GenBank数据库，并进行BLAST比对。以肝片吸虫为外群，用MEGA软件采用邻接法构建系统进化树。共采集溪蟹71只，感染并殖吸虫囊蚴60只，感染率为84.51%。检获囊蚴782个，感染度为13个/只。PCR扩增条带长约500 bp。BLAST比对结果显示，本研究获得的ITS2序列（GenBank登录号：PQ510326.1）与吉林、上海、浙江的卫氏并殖吸虫（GenBank登录号：AB713404.1、KC417492.1、JQ354935.1）的序列一致性分别为99.13%、98.79%、99.18%。系统进化树分析结果显示，本研究获得的2条序列与吉林、上海、浙江的卫氏并殖吸虫（GenBank登录号：AB713404.1、KC417492.1、JQ354935.1）的遗传距离均为0，并聚在一个分支上，与斯氏并殖吸虫（GenBank登录号：AB325516.1、AB703444.1）、宮崎并殖吸虫（GenBank登录号：AB713405.1、AY618741.1）的遗传距离分别为0.079、0.075、0.078、0.078，并形成明显的树枝分节。本次采集的溪蟹卫氏并殖吸虫感染率较高，与其他省份的卫氏并殖吸虫序列一致性较高，可作为福建省卫氏并殖吸虫的参比株。

关键词: 并殖吸虫, 核糖体内转录间隔区2, 基因分型, 系统发育, 福建省

Abstract:

To investigate the prevalence of Paragonimus metacercariae infections and genotyping of Paragonimus metacercariae in freshwater crabs from Jianning County, Fujian Province, freshwater crabs were collected from mountain streams and ditches near residential areas along the Chuxi River system in Jianning County, Fujian Province, and Paragonimus metacercariae were collected by twice filtering through the sieves for morphological characterization. Genomic DNA was extracted from Paragonimus metacercariae, and the ribosomal internal transcribed spacer 2 (ITS2) gene sequence was amplified using PCR assay. Following sequencing of the PCR amplification product, the gene sequence was uploaded to the GenBank database, and gene sequence alignment was performed using the BLAST tool. A phylogenetic tree was built using the neighbor-joining method with the MEGA software with Fasciola hepatica as an outgroup. A total of 71 freshwater crabs were captured, and there were 60 crabs infected with Paragonimus metacercariae, with an 84.51% prevalence rate of infections. A total of 782 Paragonimus metacercariae were identified, and the intensity of infections was 13 metacercariae per crab. The PCR amplification product were approximately 500 bp in length. The ITS2 gene sequence from this study (GenBank accession No.: PQ510326.1) was 99.13%, 98.79%, and 99.18% identical to P. westermani isolates from Jilin Province (GenBank accession No.: AB713404.1), Shanghai Municipality (GenBank accession No.: KC417492.1), and Zhejiang Province (GenBank accession No.:JQ354935.1), respectively. Phylogenetic analysis revealed that the two gene sequences obtained from this study were clustered into the same clade with sequences of P. westermani isolates from Jilin Province (GenBank accession No.:AB713404.1), Shanghai Municipality (GenBank accession No.: KC417492.1), and Zhejiang Province (GenBank accession No.: JQ354935.1) at a genetic distance of 0, and they exhibited genetic distances of 0.079 and 0.075 P. skrjabini isolates (GenBank accessions No.: AB325516.1 and AB703444.1) and 0.078, and 0.078 from P. miyazakii isolates (GenBank accessions No.: AB713405.1 and AY618741.1), with distinct clade separation. The gene sequences of P. westermani isolates from this study share high identities to those of P. westermani isolates from other provinces, suggesting that these P. westermani isolates may serve as a reference isolate of P. westermani in Fujian Province.

Key words: Paragonimus, ITS2, Genotyping, Phylogeny, Fujian Province

中图分类号:

R532.221

高澜琳, 谢汉国, 江典伟, 蔡武卫, 郑丹. 福建省建宁县溪蟹并殖吸虫囊蚴感染情况及基因分型[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(3): 436-439.

GAO Lanlin, XIE Hanguo, JIANG Dianwei, CAI Wuwei, ZHENG Dan. Prevalence of Paragonimus metacercariae infection and genotyping of Paragonimus metacercariae in freshwater crabs from Jianning County, Fujian Province[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2025, 43(3): 436-439.

图/表 3

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