中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (2): 15-116.

• 实验研究 • 上一篇    下一篇

微波辐射ELISA诊断日本血吸虫病的初步研究

顾启章1,张宏莹1,朱善济1,徐文娟 2 指导者: 金国梁   

  1. 1 杭州医学高等专科学校 杭州3100122 浙江省人民医院病理科 杭州310014
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-04-30 发布日期:1999-04-30

PRELIMINARY STUDIES ON MICROWAVE IRRADIATION ELISA FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA

GU Qizhang 1;ZHANG Hongying 1;ZHU Shanji 1;XU Wenjuan 2 Director: JIN Guoliang 1
  

  1. Hangzhou Medical College;Hangzhou 310012 2 Department of Pathology;Zhejiang Peoples Hospital;Hangzhou 310014
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-04-30 Published:1999-04-30

摘要:   目的:探索高效快速的血吸虫病诊断方法。方法:用微波辐射ELISA和快速ELISA同步检测各期日本血吸虫病患者血清118份,健康人血清61份,并殖吸虫病犬血清12份。结果:两种方法的血吸虫病患者血清抗体阳性检出率分别为88.1%(104/118)和91.5%(108/118),无显著性差异。健康人血清抗体假阳性分别为2份(3.3%)和1份(1.6%),亦无显著性差异;交叉反应均无假阳性。结论:微波辐射ELISA具有与快速ELISA类似的敏感性和特异性,但前者具有快速获得检测结果的优点。

关键词: 血吸虫病, 血清学诊断, 抗体检测, 微波辐射ELISA

Abstract:  AIM: To explore a fast and highly efficient method for the diagnosis of schistosomiasis japonica. METHODS: Using microwave irradiation ELISA(MWI ELISA)and fast ELISA to detect specific antibodies in sera from 118 cases with schistosomiasis japonica, 61 healthy individuals and 12 paragonimiasis cases. RESULTS: The positive rates of schistosomiasis cases were 88 1% (104/118) by MWI ELISA and 91 5% (108/118) by fast ELISA, respectively (χ 2=0 74, P>0 05). The false positive reaction of healthy individuals was found in 2 cases(3 3%) by MWI ELISA and 1 case (1 6%) by fast ELISA, respectively (χ 2=0 34, P>0 05). No false positive reaction was found in paragonimiasis cases. CONCLUSION: The sensitivity and specificity of the two tests were similar, however, MWI ELISA was faster than fast ELISA.

Key words: Schistosomiasis, serological diagnosis, antibody detection, microwave irradiation ELISA