免疫刺激DNA序列对粉尘螨诱导的Th1和Th2细胞因子的调节作用

中国寄生虫学与寄生虫病杂志 ›› 2001, Vol. 19 ›› Issue (2): 1-67.

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免疫刺激DNA序列对粉尘螨诱导的Th1和Th2细胞因子的调节作用

周承,姚航平,温廷桓,孙翠如

浙江大学医学院附属第一医院传染病研究所!杭州310003(周承;姚航平;孙翠如);上海医科大学医学螨类研究室!上海200032(温廷桓)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-04-30 发布日期:2001-04-30

Effect of Immunostimulatory DNA Sequence on the Production of Th1 and Th2 Cytokines Induced by Dermatophagoides farinae Allergen in vitro

ZHOU Cheng ¹;YAO Hang ping ¹;WEN Ting huan ²;SUN Cui ru ¹

1 Institute of Infectious Diseases;First Affiliated Hospital of School of Medicine;Zhejiang University;Hangzhou 310003; 2 Medical Acarology Laboratory;Shanghai Medical University,shanghai 200032

Received:1900-01-01 Revised:1900-01-01 Online:2001-04-30 Published:2001-04-30

摘要/Abstract

摘要： 　　目的　研究免疫刺激DNA序列 (ISS)对螨性过敏性哮喘患者外周血单个核细胞 (PBMC)经粉尘螨变应原 (Df)诱导的Th1和Th2细胞因子的调节效应。方法　分离螨性过敏性哮喘患者及正常人PBMC ,加入ISS和Df刺激培养 ;以酶联免疫吸附试验检测培养上清中IFN γ、IL 12及IL 5含量 ;用荧光免疫酶标法检测患者血清中Df特异性IgE ,并作统计相关性分析。结果　无论正常人还是患者PBMC加入ISS和Df刺激培养后 ,其上清中IL 12、IFN γ的含量较Df和对照序列刺激组明显增高 ,而IL 5却明显降低。正常对照组PBMC经ISS及Df刺激后产生的IFN γ、IL 12水平明显高于患者组 ,而IL 5则相反。患者组PBMC经ISS及Df刺激后产生的IL 12与IFN γ呈明显正相关。结论　ISS对正常人及螨性变态反应患者PBMC具有显著增强尘螨变应原诱导的Th1细胞因子表达 ,并抑制Th2细胞因子产生的作用 ,在尘螨变态反应疾病的治疗中具有潜在的临床价值。

关键词: 免疫刺激DNA序列, 粉尘螨, 哮喘, 细胞因子

Abstract: 　Objective To invesigate the immunoregulatory effect of immunostimulatory DNA sequence (ISS ) on the production of Th1 and Th2 cytokines induced by mite allergen in PBMC of the patients with mite allergic asthma in vitro . Methods PBMC from the patients with allergic asthma and normal controls were isolated and cultured in vitro stimulated by ISS and Dermatophagoides farinae allergen (Df). IL 12, IFN γ and IL 5 in the cell supernatants were detected by ELISA. Df specific IgE in sera of patients were assayed by fluorescent enzyme immunoassay. Results PBMC from both the patients and normal controls stimulated by ISS plus Df produced a significant increase in the level of both IFN γ and IL 12 compared with non ISS and Df stimulations, whereas IL 5 was decreased. Moreover, the levels of IFN γ, IL 12 produced were significantly higher in normal controls than in the patients, on the contrary, IL 5 was down regulated. It was also shown that the level of IL 12 produced by PBMC of the patients with ISS plus Df stimulation correlated positively with that of IFN γ. Conclusion ISS not only promotes the expressions of Th1 cytokines but also downregulates the production of Th2 cytokines induced by Df in both allergic and non allergic individuals, indicating its potential application in the immunotherapy of mite allergy.

Key words: immunostimulatory DNA sequence, Dermatophagoides farinae, asthma, cytokine

周承;姚航平;温廷桓;孙翠如. 免疫刺激DNA序列对粉尘螨诱导的Th1和Th2细胞因子的调节作用[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(2): 1-67.

ZHOUCheng;YAOHangping;WENTinghuan;SUNCuiru. Effect of Immunostimulatory DNA Sequence on the Production of Th1 and Th2 Cytokines Induced by Dermatophagoides farinae Allergen in vitro[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2001, 19(2): 1-67.

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免疫刺激DNA序列对粉尘螨诱导的Th1和Th2细胞因子的调节作用

Effect of Immunostimulatory DNA Sequence on the Production of Th1 and Th2 Cytokines Induced by Dermatophagoides farinae Allergen in vitro

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