海南省五指山猪毕氏肠微孢子虫和隐孢子虫的感染情况与基因型鉴定

doi:10.12140/j.issn.1000-7423.2025.05.011

中国寄生虫学与寄生虫病杂志 ›› 2025, Vol. 43 ›› Issue (5): 670-677.doi: 10.12140/j.issn.1000-7423.2025.05.011

海南省五指山猪毕氏肠微孢子虫和隐孢子虫的感染情况与基因型鉴定

董亚童¹^,²(), 张艳²^,^*(), 刘光亮², 曹宗喜², 余淼², 陈琳琳², 刘海隆², 陈素贞²

1 新疆农业大学动物医学学院，新疆乌鲁木齐 830052
2 海南省农业科学院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室/夏咸柱院士团队创新中心，海南海口 571100

收稿日期:2025-06-20 修回日期:2025-09-12 出版日期:2025-10-30 发布日期:2025-10-27
通讯作者: *张艳，女，博士，研究员，主要从事动物疫病防治研究。E-mail：zy79818_0@163.com
作者简介:董亚童，男，硕士研究生，主要从事动物疫病防治研究。E-mail：1781021457@qq.com
基金资助:
国家重点研发项目(2023YFC3404302)

Infection and genotype identification of Enterocytozoon bieneusi and Cryptosporidium in Wuzhishan pigs of Hainan

DONG Yatong¹^,²(), ZHANG Yan²^,^*(), LIU Guangliang², CAO Zongxi², YU Miao², CHEN Linlin², LIU Hailong², CHEN Suzhen²

1 College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
2 Institute of Animal Science and Veterinary Medicine of Hainan Academy of Agricultural Sciences, Key Laboratory of Tropical Animal Breeding and Disease Research, XIA Xianzhu Academician Team Innovation Center, Haikou 571100, Hainan, China

Received:2025-06-20 Revised:2025-09-12 Online:2025-10-30 Published:2025-10-27
Contact: *Email: zy79818_0@163.com
Supported by:
National Key Research and Development Program of China(2023YFC3404302)

摘要/Abstract

摘要：

目的分析海南省五指山猪感染的隐孢子虫虫种和毕氏肠微孢子虫基因型，掌握两种原虫的感染情况。方法 2024年6月—2025年2月，从海南省遵谭镇、琼中黎族苗族自治县、五指山市、澄迈县的4个养殖场（分别标记为A、B、C、D场）采集五指山猪新鲜粪样。提取粪样DNA，巢式PCR扩增隐孢子虫18S核糖体RNA（18S rRNA）和毕氏肠微孢子虫内转录间隔区（ITS）序列，阳性扩增产物测序后进行同源性比对，鉴定隐孢子虫虫种或毕氏肠微孢子虫基因型。采用SPSS 26.0软件进行统计学分析，阳性率差异的比较采用皮尔逊卡方（χ²）检验，并计算风险系数（OR）和95%置信区间（CI）。采用MEGA 11软件邻接法构建系统进化树。结果共采集137份五指山猪粪样，其中有41份扩增出830 bp的隐孢子虫18S rRNA序列，69份扩增出390 bp的毕氏肠微孢子虫ITS序列。4个养殖场的五指山猪隐孢子虫总阳性率为29.9%（41/137），其中C场最高（64.3%，18/28）、B场最低（20.3%，14/69）。断奶后仔猪的隐孢子虫阳性率为57.1%（12/21），高于断奶前仔猪的7.7%（2/26）（χ² = 13.583，P < 0.05）；断奶后仔猪和成年猪的感染OR是断奶前仔猪的16.00倍（95% CI = 2.98~85.99）和5.14倍（95% CI = 1.15~23.31）。毕氏肠微孢子虫总阳性率为50.4%（69/137），其中C场最高（60.7%，17/28）、A场最低（37.5%，9/24）。断奶后仔猪的毕氏肠微孢子虫阳性率为80.9%（17/21），高于断奶前仔猪的42.3%（11/26）和成年猪的45.5%（41/90）（χ² = 7.204、8.551，均P < 0.01）；断奶后仔猪和成年猪的感染OR是断奶前仔猪的5.80倍（95% CI = 1.52~22.10）和1.14倍（95% CI = 0.47~2.76）。放牧五指山猪的毕氏肠微孢子虫阳性率为62.8%（44/70），高于舍饲五指山猪的37.3%（25/67）（χ² = 8.935，P < 0.01）；放牧五指山猪的感染OR是舍饲五指山猪的2.84倍（95% CI = 1.42~5.69）。序列比对结果显示，41条隐孢子虫18S rRNA序列均鉴定为猪隐孢子虫，与种猪隐孢子虫（C. scrofarum，GenBank登录号：PQ856498）的序列一致性达93.36%~99.74%；在系统进化树中与云南猪源种猪隐孢子虫、湖北猪源种猪隐孢子虫（GenBank登录号：PQ856498、ON149807）处于同一大分支。69条毕氏肠微孢子虫ITS序列鉴定为H、D、和EpbC 3种基因型，其中3条H基因型序列与德国猪源H基因型（GenBank登录号：AF135835.1）的一致性达98.00%，25条D基因型序列与澳大利亚猫源D基因型（GenBank登录号：MK696083.1）的一致性达97.51%~99.72%，41条EpbC基因型序列与中国熊猫源EpbC基因型（GenBank：登录号KY950535.1）的一致性达98.59%~99.73%；系统进化树中，D基因型与阿根廷人源D基因型和澳大利亚猫源D基因型（GenBank登录号：OP650902、MK696083）处于同一分支，EbpC基因型与中国熊猫源EpbC基因型（GenBank登录号：KY950535.1）处于同一分支，H基因型与德国猪源H基因型（GenBank登录号：AF135835.1）处于同一分支。种系发育分析显示3个基因型均属于1组亚群。结论海南五指山猪存在毕氏肠微孢子虫和隐孢子虫感染，隐孢子虫感染虫种为种猪隐孢子虫，毕氏肠微孢子虫主要基因型为H、D、EbpC。

关键词: 毕氏肠微孢子虫, 隐孢子虫, 五指山猪, 人兽共患病, 基因型

Abstract:

Objective To analyze the species of Cryptosporidium and the genotype of Enterocytozoon bieneusi in Wuzhishan pigs in Hainan, and to master the infection of the two protozoa. Methods From June 2024 to February 2025, fresh fecal samples of Wuzhishan pigs were collected from 4 farms in Zuntan Town, Qiongzhong Li and Miao Autonomous County, Wuzhishan City and Chengmai County of Hainan Province (marked as A, B, C and D farms, respectively). DNA was extracted from fecal samples, and the 18S ribosomal RNA (18S rRNA) of Cryptosporidium and the internal transcribed spacer (ITS) sequence of E. bieneusi were amplified by nested PCR. The positive amplification products were sequenced and compared to identify Cryptosporidium species or E. bieneusi genotypes. SPSS 26.0 software was used for statistical analysis. Pearson chi-square (χ²) test was used to compare the differences in positive rates, and the risk coefficient (OR) and 95% confidence interval (CI) were calculated. The phylogenetic tree was constructed by MEGA 11 software adjacency method. Results A total of 137 Wuzhishan pig manure samples were collected, of which 41 were amplified with 830 bp of Cryptosporidium 18S rRNA sequence and 69 were amplified with 390 bp of E. bieneusi ITS sequence. The total positive rate of Cryptosporidium in Wuzhishan pigs from four farms was 29.9% (41/137), with the highest in C farm (64.3%, 18/28) and the lowest in B farm (20.3%, 14/69). The positive rate of Cryptosporidium in post-weaning piglets was 57.1% (12/21), which was higher than 7.7% (2/26) in pre-weaning piglets (χ² = 13.583, P < 0.05). The OR of post-weaning piglets and adult pigs was 16.00 times (95% CI = 2.98-85.99) and 5.14 times (95% CI = 1.15-23.31) higher than that of pre-weaning piglets. The total positive rate of E. bieneusi was 50.4% (69/137), with the highest in C farm (60.7%, 17/28) and the lowest in A farm (37.5%, 9/24). The positive rate of E. bieneusi in post-weaning piglets was 80.9% (17/21), which was higher than that in pre-weaning piglets (42.3%, 11/26) and adult pigs (45.5%, 41/90) (χ² = 7.204, 8.551; both P < 0.01). The OR of post-weaning piglets and adult pigs was 5.80 times (95% CI = 1.52-22.10) and 1.14 times (95% CI = 0.47-2.76) higher than that of pre-weaning piglets. The positive rate of E. bieneusi in grazing Wuzhishan pigs was 62.8% (44/70), which was higher than that in barn feeding Wuzhishan pigs (37.3%, 25/67) (χ² = 8.935, P < 0.01). The OR of infection in grazing Wuzhishan pigs was 2.84 times (95% CI = 1.42-5.69) higher than that in feeding Wuzhishan pigs. The results of sequence alignment showed that 41 Cryptosporidium 18S rRNA sequences were identified as C. scrofarum, and the sequence identity with C. scrofarum (GenBank accession number: PQ856498) was 93.36%-99.74%. In the phylogenetic tree, it was in the same large branch with C. scrofarum from Yunnan pig and Hubei pig (GenBank accession number: PQ856498, ON149807). 69 ITS sequences of E. bieneusi were identified as H, D, and EpbC genotypes. Among them, 3 H genotype sequences were 98.00% identical to the H genotype of German pig (GenBank accession number: AF135835.1). The identity of 25 D genotype sequences with D genotype (GenBank accession number: MK696083.1) from Australian cats was 97.51%-99.72%, and the identity of 41 EpbC genotype sequences with EpbC genotype (GenBank accession number: KY950535.1) from Chinese pandas was 98.59%-99.73%. In the phylogenetic tree, D genotype was in the same branch with Argentinean D genotype and Australian cat D genotype (GenBank accession number: OP650902, MK696083), EbpC genotype was in the same branch with Chinese panda EpbC genotype (GenBank accession number: KY950535.1), and H genotype was in the same branch with German pig H genotype (GenBank accession number: AF135835.1). Phylogenetic analysis showed that the three genotypes belonged to one subgroup. Conclusion There were E. bieneusi and Cryptosporidium infection in Wuzhishan pigs in Hainan. The Cryptosporidium infection species was C. scrofarum. The main genotypes of E. bieneusi were H, D and EbpC.

Key words: Enterocytozoon bieneusi, Cryptosporidium, Wuzhishan pig, Zoonotic diseases, Genotype

中图分类号:

R382.3

董亚童, 张艳, 刘光亮, 曹宗喜, 余淼, 陈琳琳, 刘海隆, 陈素贞. 海南省五指山猪毕氏肠微孢子虫和隐孢子虫的感染情况与基因型鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(5): 670-677.

DONG Yatong, ZHANG Yan, LIU Guangliang, CAO Zongxi, YU Miao, CHEN Linlin, LIU Hailong, CHEN Suzhen. Infection and genotype identification of Enterocytozoon bieneusi and Cryptosporidium in Wuzhishan pigs of Hainan[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2025, 43(5): 670-677.

图/表 5

表1

图1

表2

图2

图3

参考文献 32

[1]	薛博文. 三种致腹泻肠道原虫多重检测方法的建立及抗隐孢子虫药物的初步筛选[D]. 长春: 吉林农业大学, 2022: 1-5.
	Xue BW. Establishment of a multiple detection method for three diarrhea-causing intestinal protozoa and preliminary screening of anti-Cryptosporidium drugs[D]. Changchun: Jilin Agricultural University, 2022: 1-5. (in Chinese)
[2]	莫全, 邓宇. 猪隐孢子虫、贾第虫及毕氏肠微孢子虫的研究进展[J]. 中国畜禽种业, 2019, 15(7): 133-135.
	Mo Q, Deng Y. Research progress of Cryptosporidium suis, Giardia and Enterocytozoon bieneusi[J]. Chin Livest Poult Breed, 2019, 15(7): 133-135. (in Chinese)
[3]	Wang RJ, Qiu SX, Jian FC, et al. Prevalence and molecular identification of Cryptosporidium spp. in pigs in Henan, China[J]. Parasitol Res, 2010, 107(6): 1489-1494.
[4]	He W, Lai C, Yang FX, et al. Comparative study of two insulinlike proteases in Cryptosporidium parvum[J]. Microorganisms, 2021, 9(4): 861.
[5]	张盈. 新疆部分规模化猪场毕氏肠微孢子虫的检测与鉴定[D]. 阿拉尔: 塔里木大学, 2020: 10-13.
	Zhang Y. Molecular identification of Enterocytozoon bieneusi in large-scale pig farms in Xinjiang[D]. Ala’er: Tarim University, 2020: 10-13. (in Chinese)
[6]	曹婷. 五指山猪和长白猪肌肉发育差异的初步研究[D]. 海口: 海南大学, 2013: 1-7.
	Cao T. Preliminary study on the difference of muscle development between Wuzhishan pig and Landrace pig[D]. Haikou: Hainan University, 2013: 1-7. (in Chinese)
[7]	李吉涛. 五指山猪近交系矮小性分子遗传基础研究[D]. 北京: 中国农业科学院, 2007: 9-11.
	Li JT. Molecular genetic basis of dwarfism in Wuzhishan pig inbred line[D]. Beijing: Chinese Academy of Agricultural Sciences, 2007: 9-11. (in Chinese)
[8]	孙瑞萍, 王峰, 魏立民, 等. 五指山猪资源保护与开发利用[J]. 中国猪业, 2013, 8(S1): 130-131.
	Sun RP, Wang F, Wei LM, et al. Protection and utilization of Wuzhishan pig resources[J]. China Swine Ind, 2013, 8(S1): 130-131. (in Chinese)
[9]	Fishman JA. Risks of infectious disease in xenotransplantation[J]. N Engl J Med, 2022, 387(24): 2258-2267.
[10]	Alves M, Xiao LH, Sulaiman I, et al. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal[J]. J Clin Microbiol, 2003, 41(6): 2744-2747.
[11]	Wang SS, Wang RJ, Fan XC, et al. Prevalence and genotypes of Enterocytozoon bieneusi in China[J]. Acta Trop, 2018, 183: 142-152.
[12]	张会军. 陕西和青海两个藏香猪场三种肠道原虫的感染情况与基因型研究[D]. 杨凌: 西北农林科技大学, 2019: 2-6.
	Zhang HJ. Prevalence and genetype of three intestinal protozoa in two Zangxiang pigfarms of Shaanxi and Qinghai Provinces[D]. Yangling: Northwest A & F University, 2019: 2-6. (in Chinese)
[13]	黎玉琼, 于有利, 郜军荣, 等. 宁夏银川地区奶牛毕氏肠微孢子虫的感染情况与基因型鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 476-479, 485.
	Li YQ, Yu YL, Gao JR, et al. Enterocytozoon bieneusi infection in dairy cows and its genotype identification in Yinchuan area of Ningxia Province[J]. Chin J Parasitol Parasit Dis, 2023, 41(4): 476-479, 485. (in Chinese)
[14]	贾丁, 但佳明, 孙燕, 等. 四川省奶牛犊牛隐孢子虫流行情况及其分子特性分析[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(4): 422-427.
	Jia D, Dan JM, Sun Y, et al. Prevalence and molecular characteristics of Cryptosporidium infection in pre-weaned dairy calves in Sichuan Province[J]. Chin J Parasitol Parasit Dis, 2019, 37(4): 422-427. (in Chinese)
[15]	Wang W, Gong QL, Zeng A, et al. Prevalence of Cryptosporidium in pigs in China: A systematic review and meta-analysis[J]. Transbound Emerg Dis, 2021, 68(3): 1400-1413.
[16]	张盈, 徐春艳, 邢金明, 等. 阿克苏某香猪场隐孢子虫和十二指肠贾第虫PCR检测[J]. 动物医学进展, 2020, 41(11): 68-71.
	Zhang Y, Xu CY, Xing JM, et al. PCR detection of Cryptosporidium spp. and Giardia duodenalis in a Xiang pig farm of Aksu[J]. Prog Vet Med, 2020, 41(11): 68-71. (in Chinese)
[17]	李蕴慧. 陕西部分规模猪场猪的三种重要肠道原虫感染情况和种群结构研究[D]. 杨凌: 西北农林科技大学, 2021: 26-30.
	Li YH. Study on occurrence and population structure of three important intestinal protozoan parasites in pigs from partial intensive pig farms in Shaanxi Province[D]. Yangling: Northwest A & F University, 2021: 26-30. (in Chinese)
[18]	石莲琴, 翟国莲, 吴杰, 等. 云南地区猪毕氏微孢子虫的分子检测及其基因型鉴定[J]. 南方农业学报, 2018, 49(8): 1654-1659.
	Shi LQ, Zhai GL, Wu J, et al. Molecular detection and genotypes identification of Enterocytozoon bieneusi in pig farms in Yunnan[J]. J South Agric, 2018, 49(8): 1654-1659. (in Chinese)
[19]	Zhao W, Zhang WZ, Yang FK, et al. High prevalence of Enterocytozoon bieneusi in asymptomatic pigs and assessment of zoonotic risk at the genotype level[J]. Appl Environ Microbiol, 2014, 80(12): 3699-3707.
[20]	张婷. 五指山猪仔猪早期断奶应激对肠道免疫性能及肠道微生物影响的研究[D]. 乌鲁木齐: 新疆农业大学, 2023: 4-6.
	Zhang T. Effects of early weaning stress on intestinal immune performance and intestinal microorganism in Wuzhishan piglets[D]. Urumqi: Xinjiang Agricultural University, 2023: 4-6. (in Chinese)
[21]	李坤. 牦牛和藏猪常见原虫调查及藏猪肺线虫线粒体基因组分析[D]. 武汉: 华中农业大学, 2019: 3-7.
	Li K. Epidemiological survey of the common protozoans infection in yaks and Tibetan pigs, and analysis of the mitochondrial genome of lungworm derived from Tibetan pig[D]. Wuhan: Huazhong Agricultural University, 2019: 3-7. (in Chinese)
[22]	李蕴慧, 张会军, 宋军科, 等. 藏香猪毕氏肠微孢子虫感染情况和基因分型[J]. 中国兽医学报, 2019, 39(9): 1788-1793.
	Li YH, Zhang HJ, Song JK, et al. Infection and genotyping of Enterocytozoon bieneusi in Zangxiang pigs[J]. Chin J Vet Sci, 2019, 39(9): 1788-1793. (in Chinese)
[23]	Zou Y, Hou JL, Li FC, et al. Prevalence and genotypes of Enterocytozoon bieneusi in pigs in Southern China[J]. Infect Genet Evol, 2018, 66: 52-56.
[24]	Li DF, Zheng SJ, Zhou CX, et al. Multilocus typing of Enterocytozoon bieneusi in pig reveals the high prevalence, zoonotic potential, host adaptation and geographical segregation in China[J]. J Eukaryot Microbiol, 2019, 66(5): 707-718.
[25]	Li W, Feng YY, Xiao LH. Diagnosis and molecular typing of Enterocytozoon bieneusi: The significant role of domestic animals in transmission of human microsporidiosis[J]. Res Vet Sci, 2020, 133: 251-261.
[26]	Zhao W, Wang Y, Xin XM, et al. Investigating Enterocytozoon bieneusi in pigs farmed in Zhejiang Province, China: Occurrence, genotype identification, evolutionary analysis, and zoonotic risk assessment[J]. Vet J, 2024, 306: 106191.
[27]	Taghipour A, Bahadory S, Khazaei S, et al. Global molecular epidemiology of microsporidia in pigs and wild boars with emphasis on Enterocytozoon bieneusi: A systematic review and meta-analysis[J]. Vet Med Sci, 2022, 8(3): 1126-1136.
[28]	Wang SS, Li JQ, Li YH, et al. Novel genotypes and multilocus genotypes of Enterocytozoon bieneusi in pigs in northwestern China: A public health concern[J]. Infect Genet Evol, 2018, 63: 89-94.
[29]	周换换. 海南岛人和动物毕氏肠微孢子虫分子遗传特征分析及人兽共患风险评估[D]. 海口: 海南医学院, 2020: 6-20.
	Zhou HH. Analysis of molecular genetic characteristics and assessment of zoonotic risk of Enterocytozoon bieneusi from humans and animals in Hainan Island[D]. Haikou: Hainan Medical University, 2020: 6-20. (in Chinese)
[30]	丁玲. 陕西省部分地区猪肠道寄生虫感染情况调查及猪源隐孢子虫种的鉴定[D]. 杨凌: 西北农林科技大学, 2015: 24-33.
	Ding L. Studies on intestinal parasite species and Cryptosporidium identification in swine in Shaanxi Province[D]. Yangling: Northwest A&F University, 2015: 24-33. (in Chinese)
[31]	Zhang WZ, Yang FK, Liu AQ, et al. Prevalence and genetic characterizations of Cryptosporidium spp. in pre-weaned and post-weaned piglets in Heilongjiang Province, China[J]. PLoS One, 2013, 8(7): e67564.
[32]	李文超, 杨回回, 阚珍珍, 等. 安徽省江北地区规模化猪场隐孢子虫感染现状调查[J]. 中国血吸虫病防治杂志, 2018, 30(4): 420-423, 464.
	Li WC, Yang HH, Kan ZZ, et al. Prevalence of Cryptosporidium spp. infections in large-scale pig farms in north of Yangtze River in Anhui Province[J]. Chin J Schisto Control, 2018, 30(4): 420-423, 464. (in Chinese)

寄生虫种 Parasite species	引物名称 Primer name	引物序列（5′→3′） Primer sequence (5′→3′)	退火温度/℃ Annealing temperature/℃	片段大小/bp Fragment size/bp
隐孢子虫	18S rRNA-F1	TTCTAGAGCTAATACATGCG	56	1 325
Cryptosporidium	18S rRNA-R1	CCCATTTCCTTCGAACAGGA
	18S rRNA-F2	GGAAGGGTTGTATTTATTAGATAAAG	58	830
	18S rRNA-R2	CTCATAAGGTGCTGAAGGAGTA
毕氏肠微孢子虫	ITS-F1	GATGGTCATAGGGATGAAGAGCTT	57	410
E. bieneusi	ITS-R1	TATGCTTAAGTCCAGGGAG
	ITS-F2	AGGGATGAAGAGCTTCGGCTCTG	55	390
	ITS-R2	AGTGATCCTGTATTAGGGATATT

特征 Feature	粪样份数 No. fecal sample	隐孢子虫 Cryptosporidium		毕氏肠微孢子虫 E. bieneusi
特征 Feature	粪样份数 No. fecal sample	阳性份数 No. positive	阳性率/% Positive rate/%	阳性份数 No. positive	阳性率/% Positive rate/%
粪样来源 Source of fecal samples
A场 Farm A	24	5	20.8（5/24）	9	37.5（9/24）
B场 Farm B	69	14	20.3（14/69）	35	50.7（35/69）
C场 Farm C	28	18	64.3（18/28）	17	60.7（17/28）
D场 Farm D	16	4	4/16	8	8/16
生长阶段 Growth stage
断奶前仔猪（≤ 60 d） Pre-weaned piglets (≤ 60 d)	26	2	7.7（2/26）	11	42.3（11/26）
断奶后仔猪（> 60~180 d） Post-weaned piglets (60-180 d)	21	12	57.1（12/21）	17	80.9（17/21）
成年猪（> 180 d） Adult pigs (> 180 d)	90	27	30.0（27/90）	41	45.5（41/90）
饲养方式 Feeding methods
舍饲 House feeding	67	17	25.4（17/67）	25	37.3（25/67）
放牧 Grazing	70	24	34.3（24/70）	44	62.8（44/70）
合计 Total	137	41	29.9（41/137）	69	50.4（69/137）

海南省五指山猪毕氏肠微孢子虫和隐孢子虫的感染情况与基因型鉴定

Infection and genotype identification of Enterocytozoon bieneusi and Cryptosporidium in Wuzhishan pigs of Hainan

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 32

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王旭, 尹建海, 韩帅, 杨扬, 李梦晴, 沈玉娟, 曹建平. 寄生虫的健康威胁与生态功能[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(4): 463-468.
[2]	崔丽君, 李腾, 沈玉娟, 张璟, 周浩, 曹建平. 肠上皮细胞cGAS-STING在抗微小隐孢子虫感染免疫中的作用机制[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(3): 311-316.
[3]	王继谱, 张玉瑾, 吴星华, 王明星, 祝玉巧, 罗辉, 高思潮, 刘欣超, 李文超. 安徽省部分地区马属动物隐孢子虫的分子检测和遗传特征[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(6): 796-801.
[4]	朱昊天, 周勇志, 曹杰, 王亚楠, 张厚双, 徐前明, 周金林. 长角血蜱天冬氨酸蛋白水解酶8基因的鉴定及其抵抗田鼠巴贝虫感染作用的研究[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(4): 433-438.
[5]	周珊珊, 姜岩岩, 曹建平. 我国啮齿目动物隐孢子虫感染现状[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(4): 512-520.
[6]	李静, 杨树峰, 高文伟, 刘卿, 朱兴全, 郑文斌. 山西省晋中市猪刚地弓形虫的感染情况及基因型鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(3): 367-371.
[7]	杨玲, 王龙, 王佳阳, 周坤铮, 闫宝龙, 赵威, 黄慧聪. 微小隐孢子虫GP900^829~1099蛋白的表达及其对小鼠巨噬细胞的免疫调节作用[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 55-62.
[8]	秦源, 刘华, 王雅雪, 张璟, 苏雅馨, 曹建平, 沈玉娟. 上海市宠物犬和猫毕氏肠微孢子虫和卡耶塔环孢子虫感染情况及分子特性研究[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 63-68.
[9]	黎玉琼, 于有利, 郜军荣, 刘芸芸, 李红兵, 牛晓昊. 宁夏银川地区奶牛毕氏肠微孢子虫的感染情况与基因型鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 476-479.
[10]	晁安琪, 李慧敏, 胡沁沁, 周晓农, 郭晓奎, 殷堃. 基于全健康理念的气候变化下人兽共患病防控策略[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 263-269.
[11]	秦源, 刘华, 姜岩岩, 曹建平, 沈玉娟. 宁波市腹泻患者隐孢子虫分子鉴定及基因特征分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 202-208.
[12]	王晨曦, 陈福民, 修乐山, 胡沁沁, 周晓农, 郭晓奎, 殷堃. 基于全健康理念的气候变化下人兽共患病风险监测预警体系[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 691-700.
[13]	秦源, 刘华, 沈玉娟. HIV/AIDS合并毕氏肠微孢子虫感染的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 760-766.
[14]	李腾, 沈玉娟, 崔丽君, 刘华, 胡媛, 姜岩岩, 曹建平. 长链非编码RNA NEAT1通过调控IL-8参与肠上皮细胞抗隐孢子虫反应[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(4): 487-492.
[15]	陈远才, 黄建营, 李俊强, 张龙现. 中国奶牛源微小隐孢子虫分子流行病学及其亚型分布[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(3): 278-284.