福寿螺生态适应性相关基因研究进展

摘要/Abstract

摘要：

福寿螺是国际自然保护联盟（IUCN）公布的全球100种恶性外来物种之一,严重破坏入侵地农作物生产和水生生态系统稳定,并可作为广州管圆线虫中间宿主传播疾病。福寿螺生态适应性强,在入侵地缺少天敌,一旦定殖形成稳定种群后防制十分困难。研究福寿螺生态适应性机制对科学防制福寿螺具有重要意义。与其他螺群相比,福寿螺功能基因的研究尚未得到足够的关注,关于其生态适应性分子机制的研究相对较少。本文综述了国内外关于福寿螺生态适应性机制相关功能基因的研究进展,包括热休克蛋白、海藻糖合成酶、多功能纤维素酶、蛋白质神经毒素2、细胞色素P450酶系等基因。这些研究结果部分揭示了福寿螺在温度、食物、捕食者、灭螺药物等环境条件影响下自身的适应性变化趋势,但更多内在调节机制尚有待深入研究。

关键词: 福寿螺, 生态适应性, 基因, 分子机制

Abstract:

Pomacea canaliculata is one of the 100 worst invasive alien species disclosed by the International Union for Conservation of Nature and Natural Resources (IUCN), which impairs food agriculture and the stability of aquatic ecosystem of the invaded areas, and acts as an intermediate host of Angiostrongylus cantonensis for disease transmission. This species is difficult to prevent and control once a stable colony is formed, due to the high ecological adaptability and the lack of natural enemy. Research on the mechanisms of the ecological adaptability of P. canaliculata is of great significance for the prevention and control. Compared to other species, research on the functional genes of P. canaliculata is still in the preliminary stage, and the molecular mechanisms of the ecological adaptability are far less studied. In this article we review recent findings on the genes closely related to the mechanisms of the ecological adaptability of P. canaliculata, including heat shock proteins, trehalose synthase, multi-functional enzyme gene, perivitellin-2, and cytochrome P450. These results advance our understanding of the adaptability tendency of P. canaliculata under various environmental conditions such as temperature, food, predator, and molluscicides. Further insightful studies are still needed to reveal the regulatoty mechanisms.

Key words: Pomacea canaliculata, Ecological adaptability, Gene, Molecular mechanism

中图分类号:

R383.241

茅光耀, 郭云海, 张仪, 肖宁. 福寿螺生态适应性相关基因研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(6): 666-672.

Guang-yao MAO, Yun-hai GUO, Yi ZHANG, Ning XIAO. Research progress on genes related to ecological adaptability of Pomacea canaliculata[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2018, 36(6): 666-672.

参考文献 82

[1]	潘颖瑛. 外来生物福寿螺入侵机制的初步研究[D]. 杭州: 杭州师范大学, 2009.
[2]	罗渡, 张海涛, 汪学杰, 等. 入侵生物福寿螺(Pomacea canaliculata)对鱼卵的捕食特性[C]// 中国水产学会学术年会. 2014.
[3]	房苗. 入侵种福寿螺对本地种铜锈环棱螺的影响与作用机制[D]. 上海: 上海海洋大学, 2016.
[4]	潘冬丽, 张家辉, 龙俊, 等. 福寿螺对水体环境与水体微生物的影响[J]. 中国生态农业学报, 2014, 22(1): 58-62.
[5]	张仪, 吕山. 《广州管圆线虫病诊断标准》解读[J]. 中国卫生标准管理, 2010, 1(4): 64-65.
[6]	柯雪梅, 苏畅, 王秀芹. 2010-2014年厦门市福寿螺广州管圆线虫感染状况调查[J]. 中国当代医药, 2015, 22(35): 157-159.
[7]	雷浩, 段岳磊, 黎小梅, 等. 大理洱海周边福寿螺感染广州管圆线虫调查[J]. 中国人兽共患病学报, 2010, 26(12): 1180.
[8]	吴睿珊, 章家恩, 罗颢, 等. 广东省1997-2013年农田螺害发生与防治状况分析[J]. 生态科学, 2017, 36(3): 52-60.
[9]	Rawlings TA, Hayes KA, Cowie RH, et al. The identity, distribution, and impacts of non-native apple snails in the continental United States[J]. BMC Evol Biol, 2007, 7: 97.
[10]	韩微. 福寿螺对逆境胁迫的行为反应及生理生化反应研究[D]. 南宁: 广西大学, 2012.
[11]	Chaichana R, Sumpan T.Environmental tolerance of invasive golden apple snails (Pomacea canaliculata (Lamarck,1822)) and Thai native apple snails (Pila scutata,(Mousson,1848))[J]. Trop Ecol, 2015, 56(3): 347-355.
[12]	Seuffert ME, Martín PR.Thermal limits for the establishment and growth of populations of the invasive apple snail Pomacea canaliculata[J]. Biol Invasions, 2016, 19(4): 1169-1180.
[13]	Tariq M, Waseem S, Bilal HA, et al. An overview on the small heat shock proteins[J]. Afr J Biotechnol, 2010, 9(7): 927-939.
[14]	曲凌云, 孙修勤, 相建海.扇贝血淋巴细胞中热休克蛋白70的流式细胞术检测方法: 中国, 1598583[P]. 2005.
[15]	Pelham HR.A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene[J]. Cell, 1982, 30(2): 517-528.
[16]	Subjeck JR, Sciandra JJ, Johnson RJ.Heat shock proteins and thermotolerance: a comparison of induction kinetics[J]. Br J Radiol, 1982, 55(656): 579-584.
[17]	Sabehat A, Lurie S, Weiss D.Expression of small heat-shock proteins at low temperatures. A possible role in protecting against chilling injuries[J]. Plant Physiol, 1998, 117(2): 651-658.
[18]	Xu Y, Zheng G, Dong S, et al. Molecular cloning, characterization and expression analysis of HSP60, HSP70 and HSP90 in the golden apple snail, Pomacea canaliculata[J]. Fish Shellfish Immunol, 2014, 41(2): 643-653.
[19]	郑国湾. 外来生物福寿螺热休克蛋白的克隆与其表达差异的研究[D]. 杭州: 中国计量学院, 2012.
[20]	Song HM, Mu XD, Gu DE, et al. Molecular characteristics of the HSP70 gene and its differential expression in female and male golden apple snails (Pomacea canaliculata) under temperature stimulation[J]. Cell Stress Chaperon, 2014, 19(4): 579-589.
[21]	王亚男, 王辉, 朱晓闻, 等. 温度、盐度对马氏珠母贝鳃Hsp70基因表达量的联合效应[J]. 应用生态学报, 2012, 23(12): 3467-3473.
[22]	Hofmann G, Somero G.Evidence for protein damage at environmental temperatures: seasonal changes in levels of ubiquitin conjugates and hsp70 in the intertidal mussel Mytilus trossulu[J]. J Exp Biol, 1995, 198(Pt 7): 1509-1518.
[23]	Tedengren M, Olsson B, Reimer O, et al. Heat pretreatment increases cadmium resistance and HSP 70 levels in baltic sea mussels[J]. Aquat Toxicol, 2000, 48(1): 1-12.
[24]	Wang XR, Xiao J, Sun DJ.Myocardial protective effects of electroacupuncture and hypothermia on porcine heart after ischemia/reperfusion[J]. Acupunct Electrother Res, 2003, 28(3/4): 193-200.
[25]	Giraud-Billoud M, Vega IA, Tosi ME, et al. Antioxidant and molecular chaperone defences during estivation and arousal in the South American apple snail Pomacea canaliculata[J]. J Exp Biol, 2013, 216(Pt 4): 614-622.
[26]	Tomanek L, Somero GN.Evolutionary and acclimation-induced variation in the heat-shock responses of congeneric marine snails (Genus Tegula) from different thermal habitats: implications for limits of thermotolerance and biogeography[J]. J Exp Biol, 1999, 202(Pt 21): 2925-2936.
[27]	Tomanek L, Somero G.Variation in the heat shock responses of congeneric marine snails (Genus Tegula) from different thermal habitats[J]. Comp Biochem Physiol A: Mol Integr Physiol, 1999, 124: S107.
[28]	Elbein AD, Pan YT, Pastuszak I, et al. New insights on trehalose: a multifunctional molecule[J]. Glycobiology, 2003, 13(4): 17R-27R.
[29]	廖丹, 陈雪, 张原. 海藻糖抗冻保护作用研究进展[J]. 广东农业科学, 2011, 38(18): 83-85.
[30]	Guo BT, Weng ML, Qiao LX, et al. Expression of Porphyra yezoensis TPS gene in transgenic rice enhanced the salt tolerance[J]. J Plant Breed Genet, 2014, 2(1):45-55.
[31]	刘光富, 俞晓平, 张蓬军, 等.一种福寿螺抗逆性相关基因海藻糖合成酶基因、其编码的蛋白质及其克隆方法: 中国, 201610122025.8[P]. 2016.05.18.
[32]	Domitrovic T, Palhano FL, Barja-Fidalgo C, et al. Role of nitric oxide in the response of Saccharomyces cerevisiae cells to heat shock and high hydrostatic pressure[J]. FEMS Yeast Res, 2003, 3(4): 341-346.
[33]	Parankusam S, Adimulam SS, Bhatnagar-Mathur P, et al. Nitric oxide (NO) in plant heat stress tolerance: current knowledge and perspectives[J]. Front Plant Sci, 2017, 8: 1582.
[34]	杨坤, 周晓农, 梁幼生, 等. 冬季温度变化对钉螺一氧化氮合酶的影响[J]. 中国血吸虫病防治杂志, 2003, 15(2): 93-97, 80.
[35]	杨坤, 周晓农, 余传信, 等. 不同温度对钉螺生殖腺一氧化氮合酶基因表达的影响[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(3): 140-143.
[36]	Kong W, Huang C, Chen Q, et al. Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis[J]. Fungal Genet Biol, 2012, 49(1): 15-20.
[37]	孟利娟. 一氧化氮和海藻糖对白灵侧耳高温响应抗氧化途径的影响研究[D]. 北京: 中国农业科学院, 2015.
[38]	庄总来, 周兴旺. 福寿螺纤维素酶的开发与应用[J]. 台湾海峡, 2000, 19(1): 6-10.
[39]	熊洪林, 王志坚. 福寿螺适应环境的结构和行为研究[C]//第二届中国西部动物学学术研讨会论文集.黔南民族师范学院, 2013: 56-57.
[40]	Wang J, Ding M, Li YH, et al. A monovalent anion affected multi-functional cellulase EGX from the mollusca, Ampullaria crossean[J]. Protein Expr Purif, 2003, 31(1): 108-114.
[41]	McAllister TA, Oosting SJ, Popp JD, et al. Effect of exogenous enzymes on digestibility of barley silage and growth performance of feedlot cattle[J]. Can J Anim Sci, 1999, 79(3): 353-360.
[42]	李姣. 福寿螺外源性及内源性消化纤维素因素的研究[D]. 长沙: 湖南农业大学, 2012.
[43]	程天印, 李姣. 多功能纤维素酶(EGXA)基因在福寿螺胃、肠和肝脏表达水平的测定[J]. 中国农学通报, 2013, 29(35): 53-57.
[44]	谷嵩. 福寿螺多功能纤维素酶基因egx的克隆与功能研究[D]. 北京: 中国农业科学院, 2007.
[45]	王骥, 丁明, 李燕红, 等. 福寿螺 (Ampullaria crossean)内源性多功能纤维素酶基因的克隆(英文)[J]. 生物化学与生物物理学报, 2003, 35(10): 941-946.
[46]	罗明珠, 章家恩, 胡九龙, 等. 福寿螺和田螺的纤维素酶反应体系对不同pH和温度的响应[J]. 华南农业大学学报, 2015, 36(4): 55-58.
[47]	欧阳嘉, 谢承佳, 李双, 等. 福寿螺糖苷水解酶基因核心片断的克隆及序列分析[J]. 南京林业大学学报(自然科学版), 2006, 30(2): 29-32.
[48]	谢承佳, 欧阳嘉, 李霜, 等. 福寿螺纤维素酶EGX 相关新基因SEGX 的发现与解析[C]//第一届全国化学工程与生物化工年会论文集. 南京工业大学, 2004: 101-105.
[49]	杨培周, 郭丽琼, 王艺红, 等. 福寿螺(Ampullaria crossean)mfc基因的分子克隆和序列分析[J]. 中国食品学报, 2008, 8(4): 21-27.
[50]	Jollès J, Fiala-Médioni A, Jollès P.The ruminant digestion model using bacteria already employed early in evolution by symbiotic molluscs[J]. J Mol Evol, 1996, 43(5): 523-527.
[51]	邹慧斌. 栉孔扇贝和海湾扇贝G型溶菌酶基因及栉孔扇贝硒结合蛋白基因的克隆和表达研究[D]. 青岛: 中国海洋大学, 2005.
[52]	Yang L, Cheng TY, Zhao FY.Comparative profiling of hepatopancreas transcriptomes in satiated and starving Pomacea canaliculata[J]. BMC Genetics, 2017, 18(1):18.
[53]	Gnanapavan S, Kola B, Bustin SA, et al. The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans[J]. J Clin Endocrinol Metab, 2002, 87(6): 2988.
[54]	Nakazato M, Murakami N, Date Y, et al. A role for ghrelin in the central regulation of feeding[J]. Nature, 2001, 409(6817): 194-198.
[55]	Liu X, York DA, Bray GA.Regulation of ghrelin gene expression in stomach and feeding response to a ghrelin analogue in two strains of rats[J]. Peptides, 2004, 25(12): 2171-2177.
[56]	Ngernsoungnern A, Ngernsoungnern P.Localization of ghrelin-like peptide in the gastrointestinal tract of the golden apple snail (Pomacea canaliculata) and changing of its concentration during fasting[J]. Acta Histochem, 2016, 118(3): 244-251.
[57]	刘巧, 赵柳兰, 杨淞, 等. 福寿螺卵抗逆性研究进展[J]. 生态学杂志, 2016(4): 1072-1079.
[58]	宋红梅, 胡隐昌, 牟希东, 等. 外来入侵生物福寿螺的生物学特性、危害与防治现状[J]. 广东农业科学, 2009, 36(5): 106-108, 110.
[59]	Heras H, Dreon MS, Pollero R.Egg carotenoproteins in neotropical Ampullariidae (Gastropoda : Arquitaenioglossa)[J]. Comp Biochem Physiol Part C Toxicol Pharmacol, 2007, 146(2): 158-167.
[60]	Dreon M S, Ituarte S, Heras H.The role of the proteinase inhibitor ovorubin in apple snail eggs resembles plant embryo defense against predation[J]. PLoS One 2010, 5(12): e15059.
[61]	Dreon MS, Fernández PE, Gimeno EJ, et al. Insights into embryo defenses of the invasive apple snail Pomacea canaliculata: egg mass ingestion affects rat intestine morphology and growth[J]. PLoS Negl Trop Dis, 2014, 8(6): e2961.
[62]	Dreon M, Lavarias S, Garin CF, et al. Synthesis, distribution, and levels of an egg lipoprotein from the apple snail Pomacea canaliculata (Mollusca : Gastropoda)[J]. J Exp Zool, 2002, 292(3): 323-330.
[63]	Frassa MV, Ceolín M, Dreon MS, et al. Structure and stability of the neurotoxin PV2 from the eggs of the apple snail Pomacea canaliculata[J]. Biochim Biophys Acta, 2010, 1804(7): 1492-1499.
[64]	Dreon MS, Frassa MV, Ceolín M, et al. Novel animal defenses against predation: a snail egg neurotoxin combining lectin and pore-forming chains that resembles plant defense and bacteria attack toxins[J]. PLoS One, 2013, 8(5): e63782.
[65]	Heras H, Frassa MV, Fernández PE, et al. First egg protein with a neurotoxic effect on mice[J]. Toxicon, 2008, 52(3): 481-488.
[66]	Meenakshi V R.Aestivation in the Indian apple snail Pila-Ⅰ. Adaptation in natural and experimental conditions[J]. Comp Biochem Physiol, 1964, 11(4): 379-386.
[67]	刘晓漫. 福寿螺不同地理种群抗药性及其生理生化差异研究[D]. 南宁: 广西大学, 2011.
[68]	张文领, 牟希东, 胡隐昌, 等. 福寿螺细胞色素P450基因CYP3192A1的克隆与表达分析[J]. 南方水产科学, 2017, 13(1): 66-75.
[69]	Guppy M, Withers P.Metabolic depression in animals: physiological perspectives and biochemical generalizations[J]. Biol Rev Camb Philos Soc, 1999, 74(1): 1-40.
[70]	Cropper EC, Miller MW, Tenenbaum R, et al. Structure and action of buccalin: a modulatory neuropeptide localized to an identified small cardioactive peptide-containing cholinergic motor neuron of Aplysia californica[J]. Proc Nat Acad Sci U S A, 1988, 85(16): 6177.
[71]	Adamson KJ, Wang T, Rotgans BA, et al. Genes and associated peptides involved with aestivation in a land snail[J]. Gen Comp Endocrinol, 2017, 246: 88-98.
[72]	丁昭珩, 赵劲秋, 陈芳源. 细胞色素P450酶系3A亚家族中CYP3A5的研究进展[J]. 诊断学理论与实践, 2007, 6(4): 388-390.
[73]	杨帆, 王进军. 昆虫细胞色素P450与抗药性关系研究进展[J]. 四川动物, 2008, 27(3): 460-463.
[74]	徐灵. 池蝶蚌亲环蛋白A(CyPA)基因特异性表达及多克隆抗体制备[D]. 南昌: 南昌大学, 2011.
[75]	邓瑞鹏. 对疣荔枝螺细胞色素P450酶系及其基因CYP的研究[D]. 汕头: 汕头大学, 2005.
[76]	艾均文, 薛宏, 何行健, 等. 家蚕细胞色素P450基因的研究进展[J]. 昆虫学报, 2011, 54(8): 918-926.
[77]	张文领, 胡隐昌. 福寿螺细胞色素P450基因的鉴定与表达分析[C]//中国水产学会学术年会. 2015.
[78]	王富轩, 肖述, 向志明, 等. 香港牡蛎(Crassostrea hongkongensis) Commd1基因的分子克隆及其在盐度胁迫下的表达分析[J]. 热带海洋学报, 2017, 36(1): 48-55.
[79]	Pedrini-Martha V, Niederwanger M, Kopp R, et al. Physiological, diurnal and stress-related variability of cadmium-metallothionein gene expression in land snails[J]. PLoS One, 2016, 11(3): e0150442.
[80]	林亚, 李世国, 谢莉萍, 等. 栉孔扇贝钙调素类似蛋白基因的克隆及其与钙调素基因表达特征的比较分析[J]. 水产科学, 2014, 33(11): 692-701.
[81]	Zhong J, Wang W, Yang X, et al. A novel cysteine-rich antimicrobial peptide from the mucus of the snail of Achatina fulica[J]. Peptides, 2013, 39: 1-5.
[82]	施志仪, 杨显祥. 三角帆蚌alpha-2巨球蛋白活性测定及不同组织的表达[J]. 生物技术通报, 2006(4): 115-120.