关键词: 临床样本, 寄生虫检测, 分析, 上海

Abstract:

Objective  To analyze the results of parasitic pathogen detection on clinical samples from Shanghai hospitals during 2011-2013. Methods  Samples of serum， stool， sputum， body fluid and biopsy were collected from hospitals. The etiological， serological and molecular biology methods were used to detect parasitic infection cases.  Results  During 2011-2013, a total of 16 151 clinical samples were collected. 855 parasitic infection were found from 5 939 samples by pathogen detection, belonging to 32 species, with a detection rate of 14.4%. The positive rate of Blastocystis hominis and Entamoeba histolytica was 8.3%（494/5 939） and 3.1%（186/5 939）， respectively. The rate of intestinal protozoa infection in under 20-year-old age group was higher than other age groups （P<0.05）. No significant difference was found between males and females （P>0.05）. Totally 10 212 serum samples were examined, the total antibody-positive rate was 7.1%（730/10 212）. In the 730 positive samples, 173 (23.7%), 143（19.6%), 139（19.0%), 132（18.1%), and 128（17.5%) showed positive for the antibodies against Cysticercus cellulosae， Schistosoma japonicum， Paragonimus westermani， Toxoplasma gondii and Sparganum mansoni, respectively. The main source regions of protozoal infection were Shanghai（269 cases）， Jiangsu（142 cases）， Anhui（106 cases） and Zhejiang（82 cases）. 89 cases were worm infection, the main source were Zhejiang（24 cases）， Shanghai（18 cases）， Jiangxi（11 cases）.  Conclusion  Among the samples from hospitals, the major intestinal protozoans are Blastocystis hominis and Entamoeba histolytica, and the sero-positive cases are mainly Cysticercus cellulosae and Schistosoma japonicum infection.

Key words: Clinical samples, Parasite detection, Analysis, Shanghai