中国寄生虫学与寄生虫病杂志 ›› 2010, Vol. 28 ›› Issue (1): 8-37.

• 论著 • 上一篇    下一篇

食用醋或酱油对旋毛虫肌幼虫感染性和生殖力的影响

张玺, 王中全*, 王书伟, 李灵招, 王明明, 姜鹏, 崔晶   

  1. 郑州大学医学院寄生虫学教研室, 郑州450052
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-28 发布日期:2010-02-28

Effect of Vinegar or Soy Sauce on the Infectivity and Reproductive Capacity of Trichinella spiralis Muscle Larvae

ZHANG Xi,WANG Zhong-quan*,WANG Shu-wei,LI Ling-zhao,
WANG Ming-ming,JIANG Peng, CUI Jing
  

  1. Department of Parasitology,Medical College,Zhengzhou University,Zhengzhou 450052,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-28 Published:2010-02-28

摘要: 目的 观察食用醋或酱油对旋毛虫肌幼虫感染性和生殖力的影响。 方法 140只雄性昆明小鼠随机均分为14组,分别喂饲经食用醋(总酸浓度4.5%, pH 3.05)、 食用酱油(含19.3% NaCl)和生理盐水浸泡不同时间的含有300条旋毛虫肌幼虫的小鼠肌肉(重约0.02 g),于喂饲后第7天和第42天每组各剖杀5只小鼠,分别观察肠道成虫、肌幼虫的数量和生殖力指数(RCI)。 结果 小鼠喂饲经食用醋处理3、6、12和24 h的旋毛虫肌幼虫后,其肠道成虫数分别为77、41、0和0条, RCI分别为52.48、18.45、0和0, 均显著低于生理盐水对照组肠道成虫数(分别为121、121、116和101条)和RCI (分别为159.10、124.56、73.63和42.17)(P<0.05)。小鼠喂饲经食用酱油处理12、24、36和48 h的旋毛虫肌幼虫后, 肠道成虫数(分别为79、39、3和0条)和RCI(分别为48.75、20.80、1.87和0)亦均明显低于对照组(116、101、95和89条,73.63、42.17、21.53和4.13)(P<0.05)。感染小鼠的肠道成虫数和RCI均随醋或酱油处理时间的延长而降低(P<0.05)。 结论 含旋毛虫肌幼虫的肌肉经食用醋或酱油处理后,肌幼虫的感染力和生殖力均明显下降。

关键词: 旋毛虫, 肌幼虫, 感染性, 生殖力, 食用醋, 食用酱油

Abstract: Objective To observe the effect of edible vinegar or soy sauce on the infectivity and reproductive capacity of muscle larvae of Trichinella spiralis. Methods One hundred and forty male Kunming mice were randomly divided into 14 groups (10 mice per group). Mice in each group were orally fed with 300 muscle larvae in meat (weighing 0.02 g) soaked with edible vinegar (pH 3.05, 4.5% acid), soy (19.3% NaCl) or saline (control) for different time respectively. Half of the infected mice were sacrificed on day 7 and day 42 post-infection respectively. The intestinal adult worms and muscle larvae were observed, and reproductive capacity index (RCI) was determined. Results The intestinal adult worms (77,41,0,and 0,respectively) and RCI (52.48,18.45,0,and 0,respectively) in mice fed with 300 muscle larvae treated by vinegar for 3,6,12,and 24 h were considerably lower than that in saline control (worm number 121,121,116,and 101; RCI 159.10,124.56,73.63,and 42.17) (P<0.05). The intestinal adult worms (79.00,39.00,3.40,and 0) and RCI (48.75,20.80,1.87,and 0) in mice fed with soy-treated larvae for 12,24,36,and 48 h were also significantly lower than that in saline control (worm number 116,101,95,and 89;RCI 73.63,42.17,21.53,and 4.13)(P<0.05). Trend analysis showed that the intestinal adult worms and RCI of the infected mice decreased along with the increase of vinegar- or soy-soaking time (P<0.05). Conclusion The infectivity and fecundity of T. spiralis muscle larvae decrease gradually after the treatment of edible vinegar or soy sauce.

Key words: Trichinella spiralis, Muscle larva, Infectivity, Reproductive capacity index, Vinegar, Soy