虫源性IgE依赖组胺释放因子诱导致敏肥大细胞释放组胺的研究

中国寄生虫学与寄生虫病杂志 ›› 2007, Vol. 25 ›› Issue (6): 8-473.

虫源性IgE依赖组胺释放因子诱导致敏肥大细胞释放组胺的研究

陈晓湘1,胡旭初2,徐劲2,余新炳2 *

1 广州医学院病原生物学教研室，广州 510182；2 中山大学中山医学院寄生虫学教研室，广州 510080

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-12-30 发布日期:2007-12-30
通讯作者: 余新炳

Parasite-origin IgE-dependent Histamine-releasing Factors in Inducing Histamine Release from Sensitized Mast Cells

CHEN Xiao-xiang1,HU Xu-chu2,XU Jing2,YU Xin-bing2 *

1 Department of Medical Microbiology，Guangzhou Medical College，Guangzhou 510182，China； 2 Department of Parasitology，Preclinical School of Sun Yat-sen University，Guangzhou 510080，China

Received:1900-01-01 Revised:1900-01-01 Online:2007-12-30 Published:2007-12-30
Contact: YU Xin-bing

摘要/Abstract

摘要： 目的制备日本血吸虫和华支睾吸虫IgE依赖组胺释放因子重组蛋白rSjHRF和rCsHRF，并观察两者诱导大鼠致敏肥大细胞释放组胺的功能。方法分别克隆SjHRF和CsHRF基因完整编码序列，将重组质粒分别转化大肠埃希菌BL21并诱导表达，亲和层析纯化可溶性表达的重组蛋白，将纯化的rSjHRF和rCsHRF分别与卵清蛋白变应原致敏的大鼠肺肥大细胞一起孵育，利用荧光分光光度法测定肥大细胞组胺释放量，并制备2种重组蛋白诱导肥大细胞释放组胺的剂量依赖曲线和动力学曲线。结果成功构建重组质粒pET?鄄30?鄄rSjHRF和pET?鄄30?鄄rCsHRF，并获得纯化的可溶性重组蛋白rSjHRF和rCsHRF；2种重组蛋白诱导大鼠致敏肥大细胞释放组胺均具有剂量依赖性，当浓度为150 mg/L时，rSjHRF和rCsHRF诱导致敏肥大细胞组胺平均释放率为49.78%和32.63%；2种重组蛋白诱导致敏肥大细胞组胺释放率随时间延长而增加，在反应开始后约35 min，组胺释放率达到最高。结论重组虫源性IgE依赖组胺释放因子可诱导大鼠致敏肥大细胞释放组胺，提示该蛋白可能与寄生性蠕虫感染诱导机体Ⅰ型超敏反应的发生相关。

关键词: 日本血吸虫, 华支睾吸虫, IgE依赖组胺释放因子, 肥大细胞, 组胺

Abstract: Objective To obtain the recombinant IgE-dependent histamine-releasing factors of Schistosoma japonicum and Clonorchis sinensis （rSjHRF and rCsHRF） and to study the effect of recombinant HRFs to induce histamine release from sensitized rat mast cells. Methods The complete coding regions of SjHRF and CsHRF were cloned separately， and the recombinant plasmids were respectively transformed and expressed in BL21 cells. The soluble recombinant rSjHRF and rCsHRF were purified. Aliquots of the mast cells obtained from the lungs of OVA-immunized rats were separately incubated with rSjHRF and rCsHRF and the released histamine was measured by the OPT spectrofluorometric procedure. The dose-dependent curves and the kinetics of histamine release induced by rSjHRF and rCsHRF were prepared. Results The recombinant plasmids pET-30-rSjHRF and pET-30-rCsHRF were constructed successfully and the purified soluble recombinant proteins rSjHRF and rCsHRF were obtained by affinity chromatography. rSjHRF and rCsHRF induced histamine release from sensitized mast cells in a dose-dependent manner. At the concentration of 150 mg/L， the average rate of histamine release from sensitized mast cells induced by rSjHRF and rCsHRF were 49.78% and 32.63%， respectively. Histamine release increased with prolonged reaction time and the maximal release occurred at 35min. Conclusion The recombinant parasite-originated IgE-dependent HRFs show an effect of inducing histamine release from sensitized mast cells， suggesting that this protein would play a role in type I hypersensitivity in hosts with parasitic infections.

Key words: Schistosoma japonicum, Clonorchis sinensis, IgE-dependent histamine-releasing factor, Mast cell, Histamine

陈晓湘;胡旭初;徐劲;余新炳. 虫源性IgE依赖组胺释放因子诱导致敏肥大细胞释放组胺的研究[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(6): 8-473.

CHENXiao-xiang;HUXu-chu;XUJing;YUXin-bing*. Parasite-origin IgE-dependent Histamine-releasing Factors in Inducing Histamine Release from Sensitized Mast Cells[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2007, 25(6): 8-473.

[1]	林伟娜, 邓王平, 杨颖, 李银龙, 秦志强, 洪炀, 冯婷, 吕超, 许静. 基于LAMP-CRISPR/Cas12a的日本血吸虫核酸快速检测方法的建立与初步评价[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(2): 167-174.
[2]	徐方方, 陈权, 胡媛, 曹建平. Tsc22d3在日本血吸虫感染小鼠肝脏NK细胞中的表达及对NK杀伤功能的影响[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(2): 175-180.
[3]	唐琦, 吕超, 王熙, 郭苏影, 许晓娟, 朱海, 李银龙, 林伟娜, 周新杰, 冯婷, 许静, 秦志强. 3种方法检测野鼠血吸虫感染的效果评价[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(2): 186-191.
[4]	吴银娟, 易雪晴, 李美玉, 徐安远, 武奥迅, 钟籽丰, 李学荣. 华支睾吸虫外泌体源Csi-miR-125a致肝纤维化作用及其机制[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(2): 198-204.
[5]	武奥迅, 钟籽丰, 徐安远, 潘筱雯, 易雪晴, 吴银娟, 李学荣. 华支睾吸虫miRNA在胆管癌发生发展中作用机制的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(2): 269-273.
[6]	祝雨莺, 赵甲光, 周长海, 诸廷俊, 黄继磊, 孟军, 蒋智华, 周晓农, 李石柱, 钱门宝. 2022年广西宾阳县华支睾吸虫感染状况及其影响因素[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(6): 701-714.
[7]	蔡泽政, 杨萍, 廖倩倩. 儿童并殖吸虫复杂性感染1例[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(6): 813-816.
[8]	徐国磊, 冯延叶, 胡薇. 基于RPA-CRISPR/Cas12a技术的日本血吸虫特异性核酸片段快速可视化检测方法的建立和应用评估[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(5): 608-614.
[9]	兰炜明, 曾玉凤, 邱婷婷, 周长海, 陈喆, 葛军, 曾小军, 诸廷俊. 2020—2022年江西省信丰县人群华支睾吸虫感染现状分析[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(5): 659-663.
[10]	柴应植, 陈华良, 余可根, 张轩, 王笑笑, 张家祺, 徐文婕, 阮卫. 2013—2022年浙江省华支睾吸虫感染监测结果分析[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(4): 469-474.
[11]	王居锋, 薛诗杰, 刘江永, 赖德华, 伦照荣. 我国多个地区麦穗鱼华支睾吸虫囊蚴感染情况及其鉴定分析[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(4): 475-480.
[12]	章乐生, 王旗, 汪峰峰, 朱海, 李清越, 马晓荷, 汪敏, 王毓洁, 汪天平, 操治国. 多酶恒温快速扩增技术结合荧光探针快速检测日本血吸虫基因方法的建立[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(4): 481-486.
[13]	刘柳, 张静, 李健科, 张浩, 张凤玉. 华支睾吸虫体内神经递质5-羟色胺的分布[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 78-82.
[14]	王宁, 彭晗琪, 高常哲, 程羽珩, 吕大兵. “夜逸蚴”日本血吸虫线粒体基因组特征与系统进化的关系[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(6): 699-707.
[15]	赵磊, 李佳, 莫刚, 李醇, 黄国洋, 彭小红. 华支睾吸虫感染对小鼠肝纤维化和免疫调节功能的影响[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(6): 760-765.