中国寄生虫学与寄生虫病杂志 ›› 2007, Vol. 25 ›› Issue (6): 7-468.

• 论著 • 上一篇    下一篇

广西猪人肉孢子虫实验感染研究

李锦辉,林珍,杜进发,覃业新   

  1. 广西壮族自治区疾病预防控制中心, 南宁 530028
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-12-30 发布日期:2007-12-30

Experimental Infection of Sarcocystis suihominis in Pig and Human Volunteer in Guangxi

LI Jin-hui,LIN Zhen,DU Jin-fa,QIN Ye-xin   

  1. Guangxi Regional Center for Disease Control and Prevention,Nanning 530028,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-30 Published:2007-12-30

摘要: 目的 研究确定广西是否存在人?鄄猪相互感染的肉孢子虫病。 方法 采用人-猪-人循环感染方法。取自然感染病例的粪便,用薛氏糖漂浮法收集肉孢子虫孢子囊(10 000个以上),拌入饲料,用吞食法感染猪。取含成熟肉孢子虫包囊的新鲜生猪肉,剁碎,用吞食法感染志愿者(含包囊数约71 000个以上)。观察感染后症状及肉孢子虫在宿主体内发育情况。 结果 人感染后约5 h出现腹胀,8~36 h腹泻水样便13次,呕吐4次,畏寒、发热(体温达38.5 ℃)、头昏、头痛、关节及肌肉酸痛、腰痛、上腹部隐痛,腿部抽筋,全身乏力,食欲明显减退。感染后第10天粪检查见未孢子化的孢子囊, 第12天检出孢子化的孢子囊,孢子囊平均大小为11.9(8.8~14.5)μm×9.2(7.5~12.5)μm。猪感染后第5~8天轻度厌食,疲乏无力、便秘、毛疏松,第17天恢复正常。包囊平均大小为299.2(175~575)μm×62.3(30~102.5)μm。缓殖子大小为11.5(9.5~13.5)μm×4.1(2.8~5.0)μm。人感染后第46天口服乙酰螺旋霉素(0.2 g/次,4次/d,连服15 d),服药后第30天粪检孢子囊阴转。 结论 广西存在猪-人相互感染的肉孢子虫病。

关键词: 猪人肉孢子虫, 感染, 广西

Abstract: Objective To confirm existence of Sarcocystis suihominis and possible transmission cycle between human and pigs. Methods Based on the human-pig-human infection cycle of Sarcocystis suihominis , feces of naturally infected pigs were collected and over 10 000 sporocysts were received by flotation technique, which were mixed with fodder to infect a normal pig. Fresh pork meat containing mature sarcocysts was chopped into pieces and swallowed by a volunteer (the first author of this paper) with about 71 000 sporocysts. Symptoms and development of the parasites after infection were observed. Results The volunteer showed abdominal distension in about 5 hours after infection, with watery diarrhea 13 times from the 8th to 36th hour, vomiting 4 times, chilling and fever with a temperature of 38.5℃, dizziness, headache, joint and muscle ache, epigastralgia, and anorexia. Unsporized sporocysts were found in the faces 10 days after infection and sporocysts appeared on the 12th day. The average size of sporocysts was 11.9(8.8-14.5)μm×9.2(7.5-12.5) μm. The infected pig showed a slight anorexia, fatigue, constipation, hair loosen in 5~8 days after infection, and returned normal on the 17th day. The average size of the sarcocysts was 299.2(175-575)μm×62.3(30-102.5)μm. Size of bradyzoites was 11.5(9.5-13.5)μm×4.1(2.8-5.0)μm. The volunteer was treated with acetylspiramycin for 15 days(0.2 g/time, 4 times/d) after 46 days of infection, and fecal examination turned negative 30 days later. Conclusion There is a man-pig cycle for Sarcocystis suihominis in Guangxi.

Key words: Sarcocystis suihominis, Infection, Guangxi