感染大链壶菌后致倦库蚊幼虫血淋巴细胞的观察

中国寄生虫学与寄生虫病杂志 ›› 2001, Vol. 19 ›› Issue (6): 3-332.

感染大链壶菌后致倦库蚊幼虫血淋巴细胞的观察

莫非¹;包怀恩²

贵阳医学院检验系贵阳550004 (莫非);贵阳医学院寄生虫学教研室贵阳550004(包怀恩)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-12-30 发布日期:2001-12-30

Observation on Hemocytes of Culex pipiens quinquefasciatus Larvae Infected by Lagenidium giganteum

MO Fei ¹;BAO Huai en ²

1 Department of Laboratory Science; 2 Department of Parasitology;Guiyang Medical College;Guiyang 550004

Received:1900-01-01 Revised:1900-01-01 Online:2001-12-30 Published:2001-12-30

摘要/Abstract

摘要： 　　目的　探讨致倦库蚊幼虫被大链壶菌感染后可能的免疫防御反应。方法　采用蚊血淋巴涂片 ,姬氏染色光镜检查法结合相差显微镜技术 ,对致倦库蚊幼虫的血淋巴细胞进行观察计数和显微摄影分析。结果　①大链壶菌感染后随感染时间的延长蚊幼单位体积血淋巴细胞数出现“增加、急剧增加、然后逐渐减少”的变化特点。②在感染后 4 8和 72h浆血胞和粒血胞比例明显增加 ,原血胞比例下降 ;在感染后 96及 12 0h ,原血胞比例相对增加 ,珠血胞和类绛血胞所占比例越来越大 ,浆血胞与粒血胞比例相对减少。③感染大链壶菌后 ,浆血胞发生空泡、细胞变形等变化。结论　感染大链壶菌后致倦库蚊幼虫出现血淋巴细胞数量、分类及形态的变化 ,显示大链壶菌在蚊体内诱发了一定程度的免疫防御反应 ,其原因可能是大链壶菌释放某些抗原物质或毒性物质所致

关键词: 致倦库蚊幼虫, 大链壶菌, 血淋巴细胞, 防御性免疫

Abstract: 　Objective To explore the possible defensive immunity of Culex pipiens quinquefasciatus larvae after being infected by Lagenidium giganteum . Methods To count and analyze morphologically hemocytes of the mosquito larvae with Giemsa's stain and by both phase contrast microscope and ordinary microscope. Results ① Following the prolonged duration of infection, the number of hemocytes featured as increase, intense increase and gradual decrease. ② 48 and 72 h after infection the proportion of plasmatocytes and granulocytes increased significantly, but that of prohemocytes showed a relatively slow increase, while 96 and 120 h after infection the proportion of spherulocytes and oenocytoids increased gradually and that of plasmatocyte and granulocytes showed a slow decrease. ③ After L. giganteum infection, vacuolation and morpholysis were found in plasmatocytes. Conclusion The changes of hemocytes in amount,classification and morphology after infection revealed that Lagenidium giganteum can induce some defensive immune reactions in the larvae of Culex pipiens quinquefasciatus possibly due to a release of antigens or toxic substances by the fungus.

Key words: Culex pipiens quinquefasciatus larva, Lagenidium giganteum, hemocyte, defensive immunity

莫非;包怀恩. 感染大链壶菌后致倦库蚊幼虫血淋巴细胞的观察[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(6): 3-332.

MOFei;BAOHuaien. Observation on Hemocytes of Culex pipiens quinquefasciatus Larvae Infected by Lagenidium giganteum[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2001, 19(6): 3-332.

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[7]	潘卫;包怀恩. 大链壶菌感染对致倦库蚊幼虫体内元素的影响[J]. 中国寄生虫学与寄生虫病杂志, 2002, 20(4): 9-225.
[8]	吴家红;包怀恩. 大链壶菌对致倦库蚊幼虫三种酶活性的影响[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(5): 14-311.
[9]	包怀恩,苏晓庆,郎书源,邹复珍. 大链壶菌对蚊幼虫作用的组织学观察[J]. 中国寄生虫学与寄生虫病杂志, 1996, 14(4): 303-306.
[10]	王晓勤,裘丽姝,何毅勋,毛守白. 湖北钉螺血淋巴细胞的形态及其吞噬功能[J]. 中国寄生虫学与寄生虫病杂志, 1994, 12(2): 100-103.
[11]	许锦江,O.R.Willis. 食蚊索虫寄生期幼虫在致倦库蚊体内发育情况的观察[J]. 中国寄生虫学与寄生虫病杂志, 1983, 1(4): 57-57.