中国寄生虫学与寄生虫病杂志 ›› 2001, Vol. 19 ›› Issue (2): 13-109.

• 实验报道 • 上一篇    下一篇

日本血吸虫成虫蛋白质的性别差异性研究

朱建国,林矫矫,万海保,苑纯秀,李铭,赵辅昆,周元聪,吴祥甫,蔡幼民
  

  1. 中国农业科学院上海家畜寄生虫病研究所农业部动物寄生虫学重点开放实验室!上海200232;中国农业科学院上海家畜寄生虫病研究所农业部动物寄生虫学重点开放实验室!上海200232;中国科学院上海生物化学研究所!上海200031;
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-04-30 发布日期:2001-04-30

Studies on the Sex Difference in Proteins Between Male and Female Adult Worm of Schistosoma japonicum

ZHU Jian guo 1;LIN Jiao jiao 1;WAN Hai bao 2;YUAN Chun xiu 1;LI Ming 1 ZHAO Fu kun 2;ZHOU Yuan cong 2;WU Xiang fu 2;CAI You min 1*   

  1. 1 Shanghai Institute of Animal Parasitology;Chinese Academy of Agricultural Science;Ke
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-04-30 Published:2001-04-30

摘要:   目的 研究日本血吸虫成虫蛋白质表达的性别差异性。方法 利用双向电泳技术对日本血吸虫中国大陆株雌雄虫体蛋白质进行电泳分析。结果 在 43kDa ,pI 5 .6 0~ 5 90处 ,雄虫有一条长度和宽度均超过雌虫并由多个斑点连在一起组成的条带 ,并有 3个较雌虫大的斑点 ;雌虫有 7个特异性斑点 ,且有一处较雄虫深的着色区。结论 血吸虫成虫蛋白质表达有性别差异性。

关键词: 日本血吸虫, 双向电泳, 蛋白质组学, 性别差异性

Abstract:  Objective To explore the sex difference in proteins between male and female adult worm of Schistosoma japonicum . Methods Two electrophoresis was used to analyse the difference of protein between the male and female adult worm of S. japonicum (Chinese strain). Results Two dimensional electrophoresis analysis revealed that at the site of 43 kDa and an isoelectric point (pI) of 5.60-5.90 the male worm exhibited a band carrying a number of spots and dots, being longer and wider than that exhibited by the female worm. The female worm exhibited 7 specific dots. Conclusion The sex differences in proteins between male and female adult worms of S.japonicum are significant.

Key words: Schistosoma japonicum, two dimensional electrophoresis, proteomics, sex difference