中国寄生虫学与寄生虫病杂志 ›› 1987, Vol. 5 ›› Issue (3): 170-172.

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呼吸在日本血吸虫能量代谢中的作用

黄左钺,励正康,邱云,王琴美   

  1. 中国预防医学科学院寄生虫病研究所
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1987-08-31 发布日期:2017-01-12

ROLE OF RESPIRATION IN THE ENERGY METABOLISM OF SCHISTOSOMA JAPONICVM

  • Received:2017-01-12 Revised:2017-01-12 Online:1987-08-31 Published:2017-01-12

摘要: 应用氧电极技术测定了日本血吸虫合抱虫及单性虫的呼吸速率,观察到用氟乙酰胺处理感染日本血吸虫的小鼠后,从小鼠肝脏和肠系膜中获得虫的呼吸速率明显降低,当呼吸被抑制达50%时,虫开始肝移。还提取了日本血吸虫的线粒体,测定了用琥珀酸钠作基质时线粒体的呼吸,并观察了腺苷二磷酸对呼吸的刺激和呼吸链抑制剂抗霉素A和寡霉素对日本血吸虫呼吸的抑制情况。

关键词: 日本血吸虫, 能量代谢, 呼吸速率, 线粒体呼吸, 寄生虫病研究所, 抗霉素A, 中国预防医学科学院, 有氧代谢, 寡霉素, 抑制剂

Abstract: The respiratory rates of adult male, female, as well as coupled schistosomes were determined. It was observed that the worms harvested from the liver and mesenteric veins of mouse after treating the experimental animals with fluoroacetamide (CF) did show a considerable reduction in respiration. Once the respiration was inhibited over 50%, hepatic shift of worms occurred. Using succinate as substrate, the effects of ADP, antimycin and oligomycin on the worm mitochondria was investigated, it was shown that ADP stimulated the respiration, whereas antimycin and oligomycin inhibited it.