中国寄生虫学与寄生虫病杂志 ›› 1987, Vol. 5 ›› Issue (1): 35-38.

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15种蠕虫虫卵的膜结构

孟宪钦,王松山,周文琴,王伯霞,李向印,王力,张玉英   

  1. 河北医学院寄生虫学教研组及电镜室
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1987-02-28 发布日期:2017-01-12

MEMBRANE STRUCTURE OF 15 SPECIES OF HELMINTHIC EGGS

  • Received:2017-01-12 Revised:2017-01-12 Online:1987-02-28 Published:2017-01-12

摘要: 采用二甲基亚砜(DMSO),冷冻断裂法制备样品,用扫描电镜观察了人蛔虫虫卵、猪蛔虫虫卵、蛲虫虫卵、美丽筒线虫虫卵、美洲钩虫虫卵、十二指肠钩虫虫卵、猪鞭虫虫卵、华支睾吸虫虫卵、卫氏并殖吸虫虫卵、姜片虫虫卵、肝片吸虫虫卵、日本血吸虫虫卵、猪带线虫虫卵、牛带绦虫虫卵、曼氏迭宫绦虫虫卵及猪巨吻棘头虫虫卵15种虫卵的膜结构。重点观察卵被结构、卵盖或卵盖样区及微管系统.

关键词: 膜结构, 肝片吸虫, 卵盖, 寄生虫学, 曼氏迭宫绦虫, 卵母细胞, 卫氏并殖吸虫, 受精卵, 美丽筒线虫, 外界膜

Abstract: An investigation by scanning electron microscopy was made on the eggs of parasitic helminths. The eggs were fractured by DMSO freezing etching method. The parasite species examined were Ascaris lumbricoides, Enterobius vermicularis, Gongylonema pul-chrum, Necator americanus, Ancylostama duodenale, Trichuris suis, Clonorchis sinensis,, Paragonimus westermani, Fasciolopsis buski, Fasciola hepatica, Schistosoma japonicum, Taenia solium, Taeniarhyrichus saginatus, Spirometra mansoni, and Macracanthorhynchus. hirudinaceus.The egg shell, protein layer, vitelline membrane and lipid layer varied with the species among nematodes. There is a layer of etching structure on the outer surface of the egg shell in some trematodes and cestodes. According to the thickness of the egg shell, the eggs can be divided into 3 types, i.e. (1) below 0.1 μm, including T. solium egg; (2)0.1-1μm, including the eggs of N. americanus, A. duodenale, E. vermicularis, C. sinensis and S. japonicum; (3)1.1-2 μm including fertilized eggs of A. lumbricoides, eggs of T. suis, G. pulchrum, F. buski, F. hepatica and Spirometra mansoni.The operculum at one end of the eggs in some trematodes and cestodes is a hatching area, through which the larva hatches out. It was found that in some nematodes a characteristic structure at one or both ends of the egg is also present which may also be regarded as the hatching area.The fine tubular system in the embryophore of T. soZiufn and T. saginatus possess; not only microtubes but also micro caverns. In the egg shell of E. vermicularis and S. japonicum, this system is composed of numerous single tubules. In N. americanus egg,, however, only numerous holes are present.