中国寄生虫学与寄生虫病杂志 ›› 1985, Vol. 3 ›› Issue (1): 17-20.

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致敏冻干血球微量间接血凝试验诊断血吸虫病的研究

杨赞元,刘华实,苏瑞云,何永康,龙人镜,王银波,邓长青,李抗美   

  1. 中国人民解放军163医院; 中国人民解放军163医院; 中国人民解放军163医院; 湖南省寄生虫病研究所; 湖南省益阳地区血吸虫病防治院; 湖南省沅江县血吸虫病防治院; 湖南省汉寿县血吸虫病防治院; 湖南省临湘县血吸虫病防治院
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1985-02-28 发布日期:2017-01-12

STUDIES ON INDIRECT HAEMOAGGLUTINATION TEST USING SENSITIZED LYOPHILIZED ERYTHROCYTES FOR THE DIAGNOSIS OF SCHISTOSOMIASIS

  • Received:2017-01-12 Revised:2017-01-12 Online:1985-02-28 Published:2017-01-12

摘要: 作者报道应用致敏冻干血球作微量间接血凝试验诊断血吸虫病。采用人“O”型红细胞,经戊二醛固定,鞣酸鞣化、日本血吸虫卵冷浸抗原液致敏,然后冷冻干燥。九年来,通过各省市检测4,835例血吸虫病病人,证明本法与粪检的阳性符合率为91.9~100%。本法操作简便,有较高的特异性与敏感性,适用于血吸虫病的血清学诊断检查。

关键词: 微量间接血凝试验, 血吸虫病诊断, 红细胞, 阳性符合率, 血吸虫病防治, 寄生虫病研究所, 寄生虫学, 血球, 日本血吸虫卵, 湖南省

Abstract: The authors used sensitized lypholized Schistosoma japonicum egg antigen in indirect haemoagglutination test for the diagnosis of schistosomiasis japonica. Human O group erythrocytes are fixed with glutaraldehyde, tanned with tannic acid, sensitized with S. japonicum egg antigen and lypholized. IHA test using the antigen recommended by the author was carried out in several provinces during the past nine years. A qualitative comparison of IHA test with faecal examination technique in 4,835 proven cases with schistosomiasis showed a high agreement, i.e., 91.9-100%. It is suggested that IHA test using this antigen is easy to handle and specific and sensitive enough to be employed for serodiagnosis of schistosomiasis.