棘球蚴病患者肠道菌群差异性分析

doi:10.12140/j.issn.1000-7423.2023.01.016

中国寄生虫学与寄生虫病杂志 ›› 2023, Vol. 41 ›› Issue (1): 103-107.doi: 10.12140/j.issn.1000-7423.2023.01.016

棘球蚴病患者肠道菌群差异性分析

曹得萍¹(), 毋德芳², 庞明泉²^,³, 彭小红¹, 李大宇¹, 樊海宁²^,³^,^*()

1.桂林医学院基础医学院人体寄生虫学教研室，广西桂林 541199
2.青海省包虫病重点实验室，西宁 810001
3.青海大学附属医院肝胆胰外科，西宁 810000

收稿日期:2022-04-14 修回日期:2022-10-09 出版日期:2023-02-28 发布日期:2023-02-22
通讯作者: * 樊海宁（1970-），男，硕士，主任医师，从事肝胆胰疾病外科治疗和包虫病临床研究。Email：fanhaining@medmail.com.cn
作者简介:曹得萍（1970-），女，博士，教授，从事人体寄生虫学教学和包虫病分子生物学研究，Email：qhmccdp@163.com
基金资助:
国家自然科学基金(NSFC 8196057);青海省科技厅项目(2019-SF-131)

Difference analysis of the gut microbiome in patients with echinococcosis

CAO Deping¹(), WU Defang², PANG Mingquan²^,³, PENG Xiaohong¹, LI Dayu¹, FAN Haining²^,³^,^*()

1. Department of Human Parasitology, Basic Medical College, Guilin Medical University, Guilin 541199, Guangxi, China
2. Qinghai Research Key Laboratory for Echinococcosis, Xining 810001, China
3. Department of Hepatopancreatobiliary Surgery, Affiliated Hospital of Qinghai University, Xining 810000, China

Received:2022-04-14 Revised:2022-10-09 Online:2023-02-28 Published:2023-02-22
Contact: * E-mail: fanhaining@medmail.com.cn
Supported by:
National Natural Science Foundation of China(NSFC 8196057);Qinghai Science and Technology Department Project(2019-SF-131)

摘要/Abstract

摘要：

2020年10—12月在青海大学附属医院肝胆外科收集13例棘球蚴患者（其中7例多房棘球蚴病、6例细粒棘球蚴病，为感染组）与13例护理患者健康家属（为对照组）的粪样。提取粪样DNA并测定浓度，逆转录合成cDNA文库，利用宏基因组二代测序平台进行测序。用线性判别分析统计棘球蚴病患者与健康对照组之间肠道菌群的差异性。结果显示，棘球蚴病患者与健康对照组肠道菌群属于厚壁菌门、拟杆菌群门、变形菌门及放线菌门等。多房棘球蚴组相对丰度较高的细菌是另枝菌、拟杆菌，细粒棘球蚴组相对丰度较高的细菌是另枝菌、史氏甲烷短杆菌、前庭链球菌。健康对照组相对丰度较高的细菌是粪拟杆菌、青春双歧杆菌、琥珀酸考拉杆菌。其中另枝菌和粪拟杆菌是差异较大的细菌。本研究中的差异菌群或许可成为棘球蚴病肠道微生物标志菌，值得进一步研究。

关键词: 棘球蚴病, 肠道菌群, 多样性, 宏基因组测序

Abstract:

Fecal samples of 13 patients (7 patients with alveolar echinococcosis, 6 patients with cystic echinococcosis as infected group) and 13 healthy family members caring for patients (as control group) were collected in the Department of Hepatobiliary Surgery of Qinghai University Affiliated Hospital from October to December in 2020. The fecal DNA was extracted and the concentration was determined, and the cDNA library was synthesized by reverse transcription, and then sequenced using the metagenomic next generation sequencing. The metagenome second-generation sequencing was used to observe the changes of intestinal flora. Linear discriminant analysis was used to analyse the differences in intestinal flora between echinococcosis patients and healthy controls. The results revealed that the flora with higher relative abundance in the alveolar echinococcosis group were Alistipes onderdonkii, Bacteroides dorei, A. finegoldii, and the flora with higher relative abundance in the cystic echinococcosis group were A. shahii, Methanobrevibacter smithii, Streptococcus vestibularis. In the healthy individuals group, there were high relative abundance of B. caccae, Bifidobacterium adolescentis, Phascolarctobacterium succinatutens. Among them, A. onderdonkii, A. shahii and B. caccae are more meaningful flora. The differential flora in this study may be the intestinal microbial marker of echinococcosis, which deserves further to study.

Key words: Echinococcosis, Gut Microbiome, Diversity, Metagenomic next generation sequencing

中图分类号:

R532.32

曹得萍, 毋德芳, 庞明泉, 彭小红, 李大宇, 樊海宁. 棘球蚴病患者肠道菌群差异性分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(1): 103-107.

CAO Deping, WU Defang, PANG Mingquan, PENG Xiaohong, LI Dayu, FAN Haining. Difference analysis of the gut microbiome in patients with echinococcosis[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2023, 41(1): 103-107.

图/表 2

参考文献 20

[1]	Wu ZD, Zhu XP. Human parasitology[M]. 3rd ed. Beijing: People’s Medical Publishing House, 2015: 214-216. (in Chinese)
	(吴忠道, 诸欣平. 人体寄生虫学[M]. 3版. 北京: 人民卫生出版社, 2015: 214-216.)
[2]	Wen H, Vuitton L, Tuxun T, et al. Echinococcosis: Advances in the 21st century[J]. Clin Microbiol Rev, 2019, 32(2): e00075-e00018.
[3]	Sender R. Are we really vastly outnumbered? revisiting the ratio of bacterial to host cells in humans[J]. Cell, 2016, 164(3): 337-340. doi: 10.1016/j.cell.2016.01.013 pmid: 26824647
[4]	Gui QF, Jin HL, Zhu F, et al. Gut microbiota signatures in Schistosoma japonicum infection-induced liver cirrhosis patients: a case-control study[J]. Infect Dis Poverty, 2021, 10: 43. doi: 10.1186/s40249-021-00821-8
[5]	Schroeder BO, Bäckhed F. Signals from the gut microbiota to distant organs in physiology and disease[J]. Nat Med, 2016, 22(10): 1079-1089. doi: 10.1038/nm.4185 pmid: 27711063
[6]	Schluter J, Peled JU, Taylor BP, et al. The gut microbiota is associated with immune cell dynamics in humans[J]. Nature, 2020, 588(7837): 303-307. doi: 10.1038/s41586-020-2971-8
[7]	Qin N, Yang FL, Li A, et al. Alterations of the human gut microbiome in liver cirrhosis[J]. Nature, 2014, 513(7516): 59-64. doi: 10.1038/nature13568
[8]	Zhao Y, Yang S, Li B, et al. Alterations of the mice gut microbiome via Schistosoma japonicum Ova-induced granuloma[J]. Front Microbiol, 2019, 10: 352. doi: 10.3389/fmicb.2019.00352
[9]	Expert consensus on diagnosis and treatment of hepatic cystic and alveolar echinococcosis (2019 edition)[J]. Chin J Dig Surg, 2019, 18(8): 711-721. (in Chinese)
	(中国医师协会外科医师分会包虫病外科专业委员会. 肝两型包虫病诊断与治疗专家共识(2019版)[J]. 中华消化外科杂志, 2019, 18(8): 711-721.)
[10]	Menzel P, Ng KL, Krogh A. Fast and sensitive taxonomic classification for metagenomics with Kaiju[J]. Nat Commun, 2016, 7: 11257. doi: 10.1038/ncomms11257 pmid: 27071849
[11]	Eckburg PB, Bik EM, Bernstein CN, et al. Diversity of the human intestinal microbial flora[J]. Science, 2005, 308(5728): 1635-1638. doi: 10.1126/science.1110591 pmid: 15831718
[12]	Kazemian N, Mahmoudi M, Halperin F, et al. Gut microbiota and cardiovascular disease: Opportunities and challenges[J]. Microbiome, 2020, 8(1): 36. doi: 10.1186/s40168-020-00821-0 pmid: 32169105
[13]	Wu YH. Variations in fecal microbial profiles of acute exacerbations and stable chronic obstructive pulmonary disease[J]. Life Sci, 2021, 265: 118738. doi: 10.1016/j.lfs.2020.118738
[14]	Bajaj JS. Altered profile of human gut microbiome is associated with cirrhosis and its complications[J]. J Hepatol, 2014, 60(5): 940-947. doi: 10.1016/j.jhep.2013.12.019 pmid: 24374295
[15]	Minemura M. Gut microbiota and liver diseases[J]. World J Gastroenterol, 2015, 21(6): 1691. doi: 10.3748/wjg.v21.i6.1691
[16]	Jakobsson HE, Rodríguez-Piñeiro AM, Schütte A, et al. The composition of the gut microbiota shapes the colon mucus barrier[J]. EMBO Rep, 2015, 16(2): 164-177. doi: 10.15252/embr.201439263 pmid: 25525071
[17]	Tyrrell KL. Re-assessment of phenotypic identifications of Bacteroides putredinis to Alistipes species using molecular methods[J]. Anaerobe, 2011, 17(3): 130-134. doi: 10.1016/j.anaerobe.2011.04.002 pmid: 21527349
[18]	Parker BJ, Wearsch PA, Veloo ACM, et al. The genus Alistipes: gut bacteria with emerging implications to inflammation, cancer, and mental health[J]. Front Immunol, 2020, 11: 906. doi: 10.3389/fimmu.2020.00906
[19]	Nakajima A, Sasaki T, Itoh K, et al. A soluble fiber diet increases Bacteroides fragilis group abundance and immunoglobulin A production in the gut[J]. Appl Environ Microbiol, 2020, 86(13): 405-420.
[20]	Wei B, Dalwadi H, Gordon LK, et al. Molecular cloning of a Bacteroides caccae TonB-linked outer membrane protein identified by an inflammatory bowel disease marker antibody[J]. Infect Immun, 2001, 69 (10): 6044-6054. doi: 10.1128/IAI.69.10.6044-6054.2001 pmid: 11553542

棘球蚴病患者肠道菌群差异性分析

Difference analysis of the gut microbiome in patients with echinococcosis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 20

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李奔福, 杨敬, 杨金玉, 罗瑞娟, 朱斌林, 陈泰林, 张丽娟, 李雪瑶, 严信留, 字金荣, 彭佳, 王正青, 李健雄, 蔡璇, 徐倩, 吴方伟, 杨亚明. 云南省大理州棘球蚴病流行病学调查和病例回顾性分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 573-578.
[2]	倪碧娴, 徐祥珍, 张强, 唐凤, 张嘉尧, 茅范贞, 戴洋, 刘耀宝, 曹俊. 2015—2022年江苏省棘球蚴病网络报告病例流行病学特征分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 636-639.
[3]	封晓晓, 白玛央金, 张颋, 陆豪杰, 魏黎明. 血清IgG唾液酸化修饰在肝棘球蚴病诊断中的应用研究[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 434-439.
[4]	朱爱娅, 王旭, 王江友, 王颖, 李杨, 宋珊, 耿燕, 兰子尧, 戴佳芮. 贵州省儿童多房棘球蚴病1例[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 520-523.
[5]	娆琬·托勒洪, 阿不都撒拉木·阿不力克木, 杨凌菲, 陈璐, 李钊, 贾芳, 宋涛. 超声表现诊断肝多房棘球蚴病的效果评价及因素分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 312-318.
[6]	王威, 蔡辉霞. 2020年青海省棘球蚴病监测结果[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 319-324.
[7]	蒉嫣, 薛垂召, 王旭, 刘白雪, 王莹, 王立英, 杨诗杰, 韩帅, 伍卫平, 肖宁. 2021年全国棘球蚴病防治进展[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 142-148.
[8]	马冰村, 刘玉英, 张甜甜, 雷雯, 马霄, 刘寿. 青海省棘球蚴病影响因素的病例对照研究[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 183-191.
[9]	马慧, 种世桂, 陈根, 张伶慧, 秦俊梅, 赵玉敏. 多房棘球蚴病相关细胞信号通路的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 223-227.
[10]	路伟民, 杨小涛, 朱瑛, 张鸿, 李霁伟, 王艳春. 儿童肺细粒棘球蚴病1例[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 253-256.
[11]	段红菊, 李凌杰, 吴向林, 闫芳, 齐蓉婷, 马天波. 2016—2021年宁夏细粒棘球蚴病手术患者住院费用及其影响因素分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(1): 75-80.
[12]	吴向林, 闫芳, 段红菊, 齐蓉婷, 马天波, 高建炜. 2021年宁夏多房棘球蚴病流行区犬和野外宿主感染情况[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 717-722.
[13]	刘玉英, 张甜甜, 马霄, 雷雯, 马冰村, 刘寿. 青海省成年人棘球蚴病防治知识知晓情况及影响因素分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 723-729.
[14]	安秀青, 王苗苗, 周鸿乾, 孟凯, 蔡剑平, 刘光辉, 阿吉德, 杨金煜. 肝多房棘球蚴病微血管密度的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 792-797.
[15]	郭璐, 吴小霞, 段兰利, 王冰洁, 徐宁, 阿热艾·阿哈太, 乌云花, 赵莉, 班万里, 陈云英, 余婉蓉, 刘帅, 潘星羽, 吾力江·卡马力, 徐晶, 穆尼拉·特列吾汗, 张壮志. 新疆部分地区牛、羊细粒棘球蚴感染情况调查及基因多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 603-609.