Enzyme Change in Bronchoalveolar Lavage Fluid of Pneumocystis Pneumonia Rats and the Effect of Garlicin Treatment
LUZhi-min;ZHANGYou-cheng;ZHANGJin-shun;SHIBao-lin;JIATian-jun;TANGHong-wei;LIUJin-hua
2007, 25(4):
7-303.
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【Abstract】 Objective To study the change of enzymes and effect of garlicin treatment on the change in bronchoalveolar lavage fluid(BALF) of rats with Pneumocystis carinii pneumonia (PCP). Methods Wistar rats were injected intramuscularly continually with dexamethasone to establish the rat model of PCP. The experimental rats (group A) were injected intramuscularly with garlicin at a dose of 10 mg/(kg·d) for 5 days in the 3rd, 6 th and 9th week respectively, and SMZ/TMP therapy group (B), PCP infected group (C) and normal group (D) were established as controls. Three days after the last treatment, the rats of all groups were killed and BALF was collected without contamination and enzymes AST, ALF, CHE, ALP, LDH, CK, CKMB, HBDH, AFU, 5′NT, ADA were examined. Results The ALP level in group C ［（573.41 ± 350.63）U/L］ was significantly higher than that in group D ［（210.56 ± 114.41）U/L］ （q=4.682， P<0.01）, group A ［（392.07 ± 217.57）U/L］ （q=3.851， P<0.05）， and group B ［（325.21 ± 180.65）U/L］ （q=4.380， P<0.01）; the level of CK, CKMB and 5′NT in group C ［948.94 ± 403.43、 489.47 ± 254.46 and （6.76 ± 3.11）U/L respectively］ was higher than those in group D ［426.22 ± 319.00, 213.33 ± 144.54 and （3.22 ± 1.20）U/L］ （q=4.696， 3.784， 3.812， P<0.05）; there was no significant difference in the level of AST, ALT, CHE, LDH, HBDH, AFU and ADA among the four groups(F=1.852, 0.958, 2.470, 1.423, 1.178, 1.342, 0.611， P＞0.05). Conclusions The level of ALP, CK, CKMB and 5′NT increases evidently in BALF of PCP infected rats, but the ALP level decreases distinctly after the garlicin treatment.
