A questionnaire was issued to staffs of schistosomiasis control institutions, health and family planning administrative agencies and hospitals in the schistosomiasis endemic areas of total 8 cities and 39 counties including Nanchang, Jiujiang, Shangrao, Jingdezheng, Yichun, Ganzhou, Yintan and Jian etc, to determine the implementation situation of Diagnostic Criteria for Schistosomiasis (WS 261-2006), from April to September in 2017. Database was constructed using software EPI data 3.0, and analyzed by software SPSS Statistics 20.0. The Chi square test was used to compare the accuracy rates of the answers, and the Pearson correlation coefficient was used to analyze the correlation between the accuracy and education. A total of 624 questionnaires were received and 450 were valid. The valid respondents consisted of 251 males and 199 females with age from 19 to 61 years old, in which 66.0% (297/450) person from staffs at schistosomiasis control institutions, 4.7%(21/450) person from staffs at health and family planning administrative agencies and 29.3%(132/450) from hospitals. The highest rate of people who used the diagnostic criteria all the time or frequently was at schistosomiasis control institutions (69.7%, 202/290), followed by administrative agency(66.7%, 14/21) and hospitals(25.6%, 32/125). In hospital, less medical workers used the diagnostic criteria with 29.6% (37/125) of them occasionally used and 44.8%(56/125) of them seldomly used. Since the diagnostic criteria had been implemented, there were 1.8%(8/450) people never used or promoted it, 69.3% (312/450) of them had advocated or promoted it with mean times of 1.96 ± 5.13. A total of 55.3% (249/450) institutions had dispatched people to other institutions to train them on average of 5.59 ± 18.98 times. There were 15.1%(68/450) staffs did not know the implementation of the criteria. The correct accuracy rates of knowledge of the criteria in staff groups with different educations, jobs, in different work unit, types were significant different, and there was a positive correlation between education level and accuracy rate (r = 0.97, P < 0.05). Total 82.3% (52/61) interviewers believed that the current diagnostic criteria was practical. The progessional level and the compliance of staffs are the most important favtors that effects the application of the criteria. However, it still exists some problems with the criteria, such as some criteria has not been set up clearly or strictly. The implementation of the Diagnostic Criteria for Schistosomiasis (WS 261-2006) was not good enough in the different levels of schistosomiasis control institutions in Jiangxi province. The advocate and education on the criteria should be strengthened.