CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2019 , Vol. 37 >Issue 4: 486 - 489

DOI: https://doi.org/10.12140/j.issn.1000-7423.2019.04.020

SHORT COMMUNUCATIONS

Morphological observations of Toxocara canis egg development in vitro

  • Yi-lin ZHANG ,
  • Sen-zhao ZHANG ,
  • Xiao-bin GU ,
  • Guang-you YANG ,
  • Yue XIE
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  • College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2019-07-02

  Online published: 2019-09-05

Supported by

Supported by the Sichuan International Science and Technology Innovation Cooperation/Hong Kong, Macao and Taiwan Science and Technology Innovation Cooperation Project (No. 2019YFH0065)

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Abstract

To observe the morphological changes of Toxocara canis egg development, an automatic microscopic photographing system was setup to track and record the whole process of T. canis egg development during continuous 21-day egg culture in vitro in this study. The results demonstrated that the whole development of T. canis egg in vitro was divided into 12 developmental stages including one-cell, two-cell, three-cell, four-cell, early-morula, late-morula, blastula, gastrula, pre-larva, L1 (the first stage larva), pre-L2 and L2 stages. The cell started to divide to form two-cell egg in 24 hours culture, appeared as 3-4 cells egg, morula, blastula and gastrula stages in 3-6 days. Total 22.5% of eggs developed to embryonated L1 with phototactic movements in 7 days. Total 10.67% eggs developed to infective embryonated L2 in 8 days. At 17-day post culture, the infective larva have been hatched and the whole eggshell collapsed. These data provide more information for the developmental biology of T. canis egg in vitro.

Key words： Toxocara canis; Eggs; Development; Morphological changes

Cite this article

Yi-lin ZHANG , Sen-zhao ZHANG , Xiao-bin GU , Guang-you YANG , Yue XIE . Morphological observations of Toxocara canis egg development in vitro[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019 , 37(4) : 486 -489 . DOI: 10.12140/j.issn.1000-7423.2019.04.020

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