CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2019 , Vol. 37 >Issue 1: 28 - 32

DOI: https://doi.org/10.12140/j.issn.1000-7423.2019.01.006

ORIGINAL ARTICLES

Infection status and molecular identification of Clonorchis sinensis in human population of Tengxian County, Guangxi

  • Meng XU ,
  • Wen HUANG ,
  • Shen OU ,
  • Jian-hai YIN ,
  • Sheng-kui CAO ,
  • Li-yu MENG ,
  • Jian-ping CAO ,
  • Yu-juan SHEN
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  • 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
    2 Tengxian Center for Disease Control and Prevention, Tengxian 543300, China

Received date: 2018-07-24

  Online published: 2019-03-18

Supported by

Supported by the Chinese Special Program for Scientific Research of Public Health (No. 201502021), the National Science and Technology Major Project (No. 2018ZX10713001) and the Fourth Round of Three-Year Public Health Action Plan of Shanghai, China (No. 15GWZK0101)

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Abstract

Objective To understand the infection status and identify the isolates of Clonorchis sinensis in human population in Tengxian County, Guangxi Zhuang Autonomous Region, in order to provide base information for the control of clonorchiasis. Methods Two villages from Tianping Town and Jinji Town were selected for the survey by stratified cluster random sampling method. Demographic and relative information were collected through a questionnaire survey. Fecal samples were collected from villagers with age over 3 year-old and eggs of C. sinensis in fecal samples were microscopically examined using a modified Kato-Katz technique in triplicate. The information for the distribution of C. sinensis infection in residents with different gender, age and education was analyzed. Egg-positive fecal samples were selected for DNA extraction. The fragment of internal transcribed spacer 2 (ITS2) of C. sinensis was amplified using PCR for DNA sequencing. The obtained sequences were compared with those deposited in GenBank. Results A total of 400 individuals in the two villages were enrolled for investigation, of which 208 were infected with C. sinensis. The prevalence of C. sinensis was 52.0% (208/400), majority of them were mild infection (69.2%, 144/208). The prevalence in Tianping Town (66.5%, 133/200) was higher than that in Jinji Town(37.5%, 75/200) with statistical significance (χ2 = 33.69, P < 0.01). The prevalence in males (58.7%, 131/223) was significantly higher than that in females (43.5%, 77/177) (χ2 = 9.18, P < 0.01). The highest prevalence appeared in the 30~39 years group (63.8%, 60/94). Individuals who received senior high school education and above had the highest prevalence (68.0%, 17/25). The prevalence was significantly different among the age groups (χ2 = 37.1, P < 0.01), as it was for different levels of education (χ2 = 23.02, P < 0.01). The sequence of 156 bp ITS2 fragment amplified from the DNA isolated from the fecal eggs was identical to the isolate of C. sinensis from Vietnam Da Nang (accession number: MF319655). Conclusion The overall prevalence of C. sinensis in Tengxian County was 52.0%, a heavily endemic area for clonorchiasis. The infection was preferable in males with middle age and higher education. The sequence of C. sinensis ITS2 isolated from this County was identical to that of a Vietnam Da Nang isolate.

Key words： Clonorchis sinensis; Prevalence; Cross-sectional study; Isolate; Internal transcribed spacers 2

Cite this article

Meng XU , Wen HUANG , Shen OU , Jian-hai YIN , Sheng-kui CAO , Li-yu MENG , Jian-ping CAO , Yu-juan SHEN . Infection status and molecular identification of Clonorchis sinensis in human population of Tengxian County, Guangxi[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019 , 37(1) : 28 -32 . DOI: 10.12140/j.issn.1000-7423.2019.01.006

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