CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2025 , Vol. 43 >Issue 5: 607 - 613

DOI: https://doi.org/10.12140/j.issn.1000-7423.2025.05.002

CONSENSUS AND GUIDELINE

Expert consensus on species identification of Trichinella spp. by nucleic acid method (2025)

  • ZHANG Xi ,
  • CUI Jing ,
  • LIU Ruodan ,
  • LONG Shaorong ,
  • CHEN Muxin ,
  • CHEN Jiaxu ,
  • FU Baoquan ,
  • LIU Xiaolei ,
  • LIU Mingyuan ,
  • ZHANG Yalan ,
  • ZHANG Hongwei ,
  • WANG Zhongquan
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  • 1 Department of Pathogen Biology, School of Basic Medical Sciences, Zhengzhou University, Institute of Parasitic Disease of Zhengzhou University, Zhengzhou 450001, Henan, China
    2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Disease Research), Shanghai 200025, China
    3 Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
    4 Institute of Zoonotic Diseases of Jilin University, WOAH Collaborating Centre for Food-Borne Parasites from the Asian-Pacific Region, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Disease (Jilin University), Changchun 130015, Jilin, China
    5 Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450000, Henan, China

Received date: 2025-07-02

  Revised date: 2025-07-27

  Online published: 2025-10-27

Supported by

Intergovernmental International Scientific and Technological Innovation Cooperation Projects of National Key Research and Development Program(2024YFE0199100);Preliminary Research Project on Health Standardization in the Public Health Sector of the Chinese Center for Disease Control and Prevention in 2022(BZ2022-Q006);National Science and Technology Basic Conditions Platform, National Parasite Resource Database Project(NPRC-2019-194-30)

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Abstract

The larvae of Trichinella spp. nematodes parasitize in humans and various vertebrates, which seriously endangers people’s health and animal meat food safety. There are numerous species within the Trichinella genus; and the infectivity and pathogenicity of Trichinella spp. larvae are obviously different. In recent years, new Trichinella species have been discovered consecutively. The available pathogenic examination methods(direct microscopy of biopsy muscles and artificial digestion) could not distinguish the Trichinella species. To provide reference for Trichinella taxonomic researches and the accurate diagnosis of trichinellosis, this consensus elaborate on the nucleic acid methods for the identification of Trichinella spp. based on the latest domestic and international research progress.

Key words： Trichinella; Trichinellosis; Species identification; Multiplex PCR; Restriction fragment length polymorphism

Cite this article

ZHANG Xi , CUI Jing , LIU Ruodan , LONG Shaorong , CHEN Muxin , CHEN Jiaxu , FU Baoquan , LIU Xiaolei , LIU Mingyuan , ZHANG Yalan , ZHANG Hongwei , WANG Zhongquan . Expert consensus on species identification of Trichinella spp. by nucleic acid method (2025)[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025 , 43(5) : 607 -613 . DOI: 10.12140/j.issn.1000-7423.2025.05.002

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