A 39-year-old female worker with her ancestral home in Shaanxi, had lived, studied, and worked in Golmud, Qinghai since birth until now. She was admitted to the Army 952nd Hospital of the People’s Liberation Army of China due to gallbladder stones on March 1, 2022. Ultrasound examinations showed multiple gallbladder stones, an enlarged gallbladder, and bile stasis, and CT scans displayed gallbladder stones. Routine blood tests showed a total white blood cell count of 9.59 × 10⁹/L, a neutrophil percentage of 70.8% (elevated), an eosinophil count of 0.01 × 10⁹/L (decreased), eosinophil percentage of 0.1% (decreased), alanine aminotransferase activity of 68.3 U/L (elevated), aspartate aminotransferase activity of 50 U/L (elevated), total protein of 59 g/L (decreased), and albumin of 37.7 g/L (decreased). Physical examination revealed tenderness in the upper-middle right abdomen. The patient underwent a laparoscopic cholecystectomy on March 2, and the pathological examination of the gallbladder identified Clonorchis sinensis eggs. The patient had no history of living in endemic areas and only had a habit of consuming sliced raw fish in Qinghai. Based on epidemiological history, clinical symptoms, and relevant auxiliary examination results, she was definitively diagnosed with C. sinensis infection, and was the first local case in Qinghai. The patient was cured and discharged from hospital on March 9, and was treated with praziquantel (at a total dose of 210 mg/kg, three times daily for successively 3 days). Subsequent multiple fecal examinations did not show any abnormalities, and no abnormalities were found at 3-month and 2-year follow-up visits.