CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2025 , Vol. 43 >Issue 3: 377 - 384

DOI: https://doi.org/10.12140/j.issn.1000-7423.2025.03.012

Original article

Sequencing and analysis of the complete mitochondrial genome of Rhipicephalus turanicus in Shandong Province

  • YU Lianqi ,
  • SU Hangli ,
  • WANG Shuang ,
  • WANG Zhitao ,
  • BU Cancan ,
  • LV Wenxiang ,
  • WANG Xinmei ,
  • LIU Lijuan ,
  • SHENG Zhaoan ,
  • ZHANG Benguang
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  • 1 Shandong First Medical University and Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Diseases, Jining 272033, Shandong, China
    2 School of Public Health, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan 250117, Shandong, China
    3 Department of Pathogenic Biology, School of Basic Medical Sciences, Jining Medical College, Jining 272067, Shandong, China
    4 School of Biomedical Sciences (Provincial Pharmaceutical Biotechnology Research Centre), Shandong First Medical University (Shandong Provincial Academy of Medical Sciences), Jinan 250117, Shandong, China

Received date: 2025-01-22

  Revised date: 2025-03-18

  Online published: 2025-06-09

Supported by

National Natural Scienee Foundation of China(81902096);Jining Medical College He Lin Academician New Medicine Clinical Translation Workstation Research Fund(JYHL2021MS22)

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Abstract

Objective To decipher the mitochondrial genome structure and phylogenetic relationships of Rhipicephalus turanicus and investigate the genetic differentiation among different geographical populations of R. turanicus, so as to provide data supports to species identification and phylogenetic analysis. Methods A male R. turanicus tick was collected and identified from the body surface of a domestic dog in Mapo Township, Weishan County, Jining City, Shandong Province on August 2023, and genomic DNA was extracted from the tick for second-generation sequencing on an Illumina high-throughput platform. The complete mitochondrial genome of R. turanicus was assembled using Novoplasty software and the functions of the complete mitochondrial genome were preliminarily annotated with the MITOS software. In addition, phylogenetic trees were constructed using the maximum likelihood method based on the sequences of 13 protein-coding genes (PCGs). Results Second-generation sequencing showed that the complete mitochondrial genome of R. turanicus was 14 718 bp in length (GenBank accession No.: PV416795), which was consisted of 13 PCGs, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 non-coding regions (NCRs). The complete mitochondrial genome contained overlapping regions or intergenic regions, and the base composition exhibited a clear A + T preference. The PCGs initiated with four typical start codons ATN (ATA, ATC, ATG, and ATT), and terminated with stop codons TAA, TAG, TA and T. Among the secondary structures of 22 tRNA gene, all formed a typical cloverleaf structure except for trnC, trnF, and trnS1, which lacked the dihydrouracil (DHU) arm and DHU loop, resulting in failure in formation of a complete cloverleaf structure. Phylogenetic analysis revealed that the R. turanicus sampled from Shandong Province in this study was clustered with tick populations from Beijing (GenBank accession No.: OM368330) and Xinjiang (GenBank accession No.: KY996841, NC035946, OM368326) into the same clade branch, and was grouped into a strongly supported monophyletic clade with populations from Israel (GenBank accession No.: OQ184023) and Saudi Arabia (GenBank accession No.: PP919886). The R. turanicus populations from different geographic regions were clustered into a highly supported phylogenetic clade, suggesting a high degree of genetic conservation and a tendency towards regional genetic differentiation. Conclusion The complete mitochondrial genome sequence of R. turanicus from Shandong Province has been obtained, which conforms to the mitochondrial genome characteristics of the Ixodidae family, and there is a tendency towards potential genetic differentiation among different geographic populations of R. turanicus.

Key words： Rhipicephalus turanicus; Mitochondrial genome; Phylogenetic analysis

Cite this article

YU Lianqi , SU Hangli , WANG Shuang , WANG Zhitao , BU Cancan , LV Wenxiang , WANG Xinmei , LIU Lijuan , SHENG Zhaoan , ZHANG Benguang . Sequencing and analysis of the complete mitochondrial genome of Rhipicephalus turanicus in Shandong Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025 , 43(3) : 377 -384 . DOI: 10.12140/j.issn.1000-7423.2025.03.012

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