CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2025 , Vol. 43 >Issue 3: 335 - 339

DOI: https://doi.org/10.12140/j.issn.1000-7423.2025.03.005

Original article

Genetic evolutionary characteristics for piroplasm infection in equines in Qinghai Province

  • LI Zhi ,
  • MENG Ru ,
  • HAN Yuan ,
  • YUAN Qing ,
  • HUANG Ying ,
  • DUO Hong ,
  • FU Yong
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  • 1 Key Laboratory of Animal Disease Pathogen Diagnosis and Green Prevention and Control Technology of Qinghai Province, Academy of Animal Sciences and Veterinary Medicine, Qinghai university, Xining 810016, Qinghai, China
    2 Xining Animal Disease Prevention and Control Center, Xining 810003, Qinghai, China

Received date: 2024-09-06

  Revised date: 2024-12-01

  Online published: 2025-06-17

Supported by

Natural Science Foundation Program of Qinghai Provincial Department of Science and Technology(2023-ZJ-955Q)

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Abstract

Objective To investigate the prevalence of tick-borne piroplasm infections and the genetic evolutionary characteristics in equines in selected areas of Qinghai Province, so as to provide insights into management of equine piroplasmosis in Qinghai Province. Methods The blood samples were collected from free range equines in four cities (prefectures) of Haidong, Xining, Haixi and Haibei in Qinghai Province from March 2023 to July 2024. Genomic DNA was extracted from blood samples and the 18S rRNA gene was amplified using nested PCR assay. The obtained gene sequence was subjected to homology alignment and analysis using the NCBI BLAST program. Haplotype analysis was performed using the software DNASP 6, and a haplotype network diagram was plotted using PopArt. The sequences of Theileria equi genotypes A to E and Babesia caballi genotypes A to C were selected from GenBank as reference sequences, and a phylogenetic tree was built using the maximum likelihood estimation with the software MEGA 6.0. Results The prevalence of piroplasm infections infection was 47.73% (84/176) in 176 blood samples from free-ranging equines, and the prevalence rates of T. equi and B. caballi infections were 47.16% (83/176) and 0.57% (1/176), respectively. A total of 16 haplotypes were identified in the 83 sequences of T. equi, with a haplotype diversity of 0.450 and a nucleotide diversity of 0.026 43, and H1 was the dominant haplotype of T. equi (73.49%, 61/83), followed by H5 haplotype (9.64%, 8/83), and the remaining 14 haplotypes (H2 to H4, H6 to H16) each accounted for 1.20% (1/83). Phylogenetic analysis showed that the gene sequences of T. equi haplotypes H5, H8 to H14, H16 were clustered together with T. equi genotype A (GenBank accession numbers: JX177672 and MT463613) in GenBank into the same clade, and the remaining 7 haplotypes were clustered together with T. equi genotype B (GenBank accession numbers: EU642507 and AB515310) into the same clade, while the gene sequences of B. caballi and B. caballi genotype A (GenBank accession numbers: EU642512, KJ787774, AY309995, AB734392, MH651219, MH651220 and JX049130) in GenBank were clustered into the same clade. Conclusion T. equi and B. caballi are piroplasm species infecting equines in selected areas of Qinghai Province, with a high prevalence rate of infections. The prevalent genotypes are T. equi genotypes A and B and B. caballi genotype A, with T. equi genotype B as the dominant genotype.

Key words： Piroplasma infection; Equine; Haplotype; Genetic evolution; Qinghai

Cite this article

LI Zhi , MENG Ru , HAN Yuan , YUAN Qing , HUANG Ying , DUO Hong , FU Yong . Genetic evolutionary characteristics for piroplasm infection in equines in Qinghai Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025 , 43(3) : 335 -339 . DOI: 10.12140/j.issn.1000-7423.2025.03.005

References

[1] Romero-Salas D, Solis-Cortés M, Zazueta-Islas HM, et al. Molecular detection of Theileria equi in horses from Veracruz, Mexico[J]. Ticks Tick Borne Dis, 2021, 12(3): 101671.
[2] Knowles DP, Kappmeyer LS, Haney D, et al. Discovery of a novel species, Theileria haneyi n. sp., infective to equids, highlights exceptional genomic diversity within the genus Theileria: Implications for api complexan parasite surveillance[J]. Int J Parasitol, 2018, 48(9/10): 679-690.
[3] Wise LN, Kappmeyer LS, Mealey RH, et al. Review of equine piroplasmosis[J]. J Vet Intern Med, 2013, 27(6): 1334-1346.
[4] 陈克伟, 胡哲, 李金坤. 我国马梨形虫病的流行病学特征及危险因素评估[J]. 中国预防兽医学报, 2022, 44(9): 1022.
  Chen K, Hu Z, Li J. Prevalence and molecular epidemiology of equine piroplasmosis in China: A neglected tick-borne disease[J]. Chin J Prev Vet Med, 2022, 44(9): 1022. (in Chinese)
[5] Chen KW, Hu Z, Li JK, et al. Prevalence and molecular epidemiology of equine piroplasmosis in China: A neglected tick-borne disease[J]. Sci China Life Sci, 2022, 65(2): 445-447.
[6] Criado-Fornelio A, Martinez-Marcos A, Buling-Saraña A, et al. Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe. Part Ⅱ. Phylogenetic analysis and evolutionary history[J]. Vet Parasitol, 2003, 114(3): 173-194.
[7] Bhoora R, Franssen L, Oosthuizen MC, et al. Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa[J]. Vet Parasitol, 2009, 159(2): 112-120.
[8] Kouam MK, Kantzoura V, Masuoka PM, et al. Genetic diversity of equine piroplasms in Greece with a note on speciation within Theileria genotypes (T. equi and T. equi-like)[J]. Infect Genet Evol, 2010, 10(7): 963-968.
[9] Peckle M, Pires MS, da Silva CB, et al. Molecular characterization of Theileria equi in horses from the state of Rio de Janeiro, Brazil[J]. Ticks Tick Borne Dis, 2018, 9(2): 349-353.
[10] Qablan MA, Oborník M, Petrželková KJ, et al. Infections by Babesia caballi and Theileria equi in Jordanian equids: Epidemiology and genetic diversity[J]. Parasitology, 2013, 140(9): 1096-1103.
[11] Munkhjargal T, Sivakumar T, Battsetseg B, et al. Prevalence and genetic diversity of equine piroplasms in Tov Province, Mongolia[J]. Infect Genet Evol, 2013, 16: 178-185.
[12] Manna G, Cersini A, Nardini R, et al. Genetic diversity of Theileria equi and Babesia caballi infecting horses of Central-Southern Italy and preliminary results of its correlation with clinical and serological status[J]. Ticks Tick Borne Dis, 2018, 9(5): 1212-1220.
[13] Leitwein M, Duranton M, Rougemont Q, et al. Using haplotype information for conservation genomics[J]. Trends Ecol Evol, 2020, 35(3): 245-258.
[14] Bowen BW, Grant WS. Phylogeography of the sardines (Sardinops spp.): Assessing biogeographic models and population histories in temperate upwelling zones[J]. Evolution, 1997, 51(5): 1601-1610.
[15] Yang JF, Li YQ, Liu ZJ, et al. Molecular evidence for piroplasms in wild Reeves’ muntjac (Muntiacus Reevesi) in China[J]. Parasitol Int, 2014, 63(5): 713-716.
[16] Olmeda AS, Armstrong PM, Rosenthal BM, et al. A subtropical case of human babesiosis[J]. Acta Trop, 1997, 67(3): 229-234.
[17] Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, et al. DnaSP 6: DNA sequence polymorphism analysis of large data sets[J]. Mol Biol Evol, 2017, 34(12): 3299-3302.
[18] 侯浩宾, 李海静, 张莉. 马、驴主要经济性状功能基因研究进展[J]. 中国畜牧兽医, 2018, 45(10): 2670-2680.
  Hou HB, Li HJ, Zhang L. Research advances on functional genes of main economic traits in horses and donkeys[J]. China Anim Husb Vet Med, 2018, 45(10): 2670-2680. (in Chinese)
[19] Niu QL, Luo JX, Guan GQ, et al. Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences[J]. Exp Parasitol, 2009, 121(1): 64-68.
[20] Liu AH, Guan GQ, Liu ZJ, et al. Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP)[J]. Exp Parasitol, 2010, 126(4): 476-481.
[21] Sarataphan N, Nilwarangkoon S, Tananyutthawongese C, et al. Genetic diversity of major piroplasm surface protein genes and their allelic variants of Theileria parasites in Thai cattle[J]. J Vet Med Sci, 1999, 61(9): 991-994.
[22] 苟惠天. 梨形虫及硬蜱标识基因的分析与初步应用[D]. 北京: 中国农业科学院, 2012: 92.
  Gou HT. Analysis and tentative application of molecular markers of Piroplasma and hard tick[D]. Beijing: Chinese Academy of Agricultural Sciences, 2012: 92. (in Chinese)
[23] Winchell CJ, Martin AP, Mallatt J. Phylogeny of elasmobranchs based on LSU and SSU ribosomal RNA genes[J]. Mol Phylogenet Evol, 2004, 31(1): 214-224.
[24] 周庆安, 黄胜斌, 莫胜兰, 等. 广西壮族自治区部分地区马属动物梨形虫感染的分子流行病学调查[J]. 中国兽医学报, 2022, 42(1): 78-81, 94.
  Zhou QA, Huang SB, Mo SL, et al. Investigation on molecular epidemiology of Piroplasma infection in equine animals in some parts of Guangxi Zhuang Autonomous Region[J]. Chin J Vet Sci, 2022, 42(1): 78-81, 94. (in Chinese)
[25] Scoles GA, Ueti MW. Vector ecology of equine piroplasmosis[J]. Annu Rev Entomol, 2015, 60: 561-580.
[26] Wang JM, Liu JL, Yang JF, et al. The first molecular detection and genetic diversity of Babesia caballi and Theileria equi in horses of Gansu Province, China[J]. Ticks Tick Borne Dis, 2019, 10(3): 528-532.
[27] Zhao SW, Wang H, Zhang S, et al. First report of genetic diversity and risk factor analysis of equine piroplasm infection in equids in Jilin, China[J]. Parasit Vectors, 2020, 13(1): 459.
[28] Zhang Y, ShiQY, Laven R, et al. Prevalence and genetic diversity of Theileria equi from horses in Xinjiang Uygur Autonomous Region, China[J]. Ticks Tick Borne Dis, 2023, 14(4): 102193.
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