CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2025 , Vol. 43 >Issue 1: 97 - 102

DOI: https://doi.org/10.12140/j.issn.1000-7423.2025.01.015

ORIGINAL ARTICLES

Species identification and ITS sequence analysis of Prosthogonimus in Grus japonensis

  • CAO Yu ,
  • LI Ye ,
  • JIN Zhenhua ,
  • WANG Likun ,
  • GAO Zhongyan ,
  • ZHANG Xianguang ,
  • JIANG Botao
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  • 1 Branch of Animal Husbandry and Veterinary Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005, Heilongjiang, China
    2 Heilongjiang Province Key Laboratory of Veterinary Drugs, Qiqihar 161005, Heilongjiang, China
    3 Heilongjiang Zhalong National Natural Reserve Administration, Qiqihar 161002, Heilongjiang, China
*E-mail: yu_cao2021@163.com

Received date: 2024-07-31

  Revised date: 2024-12-17

  Online published: 2025-03-26

Supported by

Heilongjiang Province Agricultural Science and Technology Innovation Leapfrog Project Agricultural Science and Technology Basic Innovation Project (Excellent Young Scholars)(CX23BS06);Doctoral Postdoctoral Funding Project in Heilongjiang Province(LBH-Z24275)

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Abstract

Objective To identify the species of Prosthogonimus in Grus japonensis and analyze the ribosomal DNA internal transcribed spacer (ITS) gene sequence. Methods Prosthogonimus samples were collected from the G. japonensis cloaca in Zhalong National Nature Reserve, Heilongjiang Province. The ITS gene of Prosthogonimus was amplified using PCR assay, and the amplified product was sequenced. The sequencing results were aligned with sequences recorded in the NCBI database using the BLAST tool. Repetitive sequences were identified using the Repeat Masker tool, and sequence similarity analysis was performed using the MegAlign software. Sequence alignments were conducted using the MEGA 7.0, Clustal X, and Paup software, and a phylogenetic tree was created using the maximum parsimony method. Results A total of 5 Prosthogonimus specimens were collected from G. japonensis, and five ITS gene sequences were obtained. Three sequences (GenBank accession numbers: PQ634967, PQ634968 and PQ634969) were 1 240 bp in size and showed 98.2% sequence similarity with P. cuneatus (GenBank accession number: OQ344776), which were identified as P. cuneatus. The other two sequences (GenBank accession numbers: PP956934 and PP956935) were 1 183 bp in size and showed 97.7% sequence similarity with P. pellucidus (GenBank accession number: KP192732), which were identified as P. pellucidus. There were no repetitive sequences in 5.8S or ITS2 genes of P. cuneatus and P. pellucidus, and there were 16 and 17 repetitive sequences the ITS1 gene of P. cuneatus and P. pellucidus, respectively. Phylogenetic analysis revealed that the P. cuneatus from this study was firstly clustered with P. cuneatus from Turdusmerula and Anasplatyrhynchos (Czech Republic isolate) into a subclade, and then clustered with P. pellucidus from this study into a clade. Conclusion The Prosthogonimus species infecting G. japonensis in the Zhalong National Nature Reserve are P. cuneatus and P. pellucidus, with 16 and 17 repetitive sequences in the P. cuneatus and P. pellucidus ITS1, respectively. P. cuneatus and P. pellucidus share a closer phylogenetic relationship with the Czech Republic isolate of P. cuneatus.

Key words： Prosthogonimus cuneatus; Prosthogonimus pellucidus; ITS sequences; Grus japonensis

Cite this article

CAO Yu , LI Ye , JIN Zhenhua , WANG Likun , GAO Zhongyan , ZHANG Xianguang , JIANG Botao . Species identification and ITS sequence analysis of Prosthogonimus in Grus japonensis[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025 , 43(1) : 97 -102 . DOI: 10.12140/j.issn.1000-7423.2025.01.015

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