CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2024 , Vol. 42 >Issue 5: 664 - 667

DOI: https://doi.org/10.12140/j.issn.1000-7423.2024.05.016

SHORT COMMUNICATIONS

Analysis of polymorphism of Pfhrp2 gene in imported Plasmodium falciparum in Henan Province in 2020

  • LI Suhua ,
  • JI Penghui ,
  • WANG Dan ,
  • WANG Hao ,
  • ZHOU Ruimin
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  • Henan Center for Disease Control and Prevention, Provincial Key Laboratory for Infectious Disease Prevention and Control, Henan Provincial Medical Key Laboratory of Parasitic Pathogen and Vector,Zhengzhou 450016, Henan, China

Received date: 2024-03-26

  Revised date: 2024-07-15

  Online published: 2024-10-28

Supported by

Joint Project of Medical Science and Technology of Henan Province of 2022(LHGJ20220157)

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Abstract

To analyze the deletion and polymorphism of exon 2 region of Plasmodium falciparum histidine rich protein 2 (Pfhrp2) gene from the blood samples of the imported falciparum malaria cases in Henan Province in 2020. The information and blood samples of the imported falciparum malaria cases confirmed by Henan Provincial Malaria Diagnosis Reference Laboratory in 2020 were collected. Nested PCR was performed to amplify Pfhrp2 exon 2 used DNA extracted from blood samples, and the positive amplification products were sequenced. A total of 37 blood samples of confirmed imported falciparum malaria cases were collected. 35 sequences of Pfhrp2 exon 2 were amplified successfully by nested PCR and the positive rates was 94.59% (35/37). 28 of 35 positive amplification products were sequenced successfully, with an average length of 822 bp. Twelve kinds of repeat types were found in the amino acid chains encoded by Pfhrp2 exon 2. All the amino acid chains started with types 1 (AHHAHHVAD) and ended with types 12 (AHHAA), and contained type 1, 2, 4, 11 and 12. The proportion of other repeat types in descending order were type 13 (96.43%, 27/28), 3 (92.86%, 26/28), 7 (82.14%, 23/28), 8 (64.29%, 18/28), 6 (60.71%, 17/28), 5 (46.43%, 13/28) and 9 (32.14%, 9/28), respectively. The results of this study showed that there was a high diversity of Pfhrp2 exon 2 in imported Plasmodium falciparum from Henan Province in 2020, which might cause different results of rapid diagnostic tests.

Key words： Imported Plasmodium falciparum; Pfhrp2 gene; Polymorphism; Henan Province

Cite this article

LI Suhua , JI Penghui , WANG Dan , WANG Hao , ZHOU Ruimin . Analysis of polymorphism of Pfhrp2 gene in imported Plasmodium falciparum in Henan Province in 2020[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024 , 42(5) : 664 -667 . DOI: 10.12140/j.issn.1000-7423.2024.05.016

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