Objective To understand the current situation of capacitity building in team development and technical needs and to propose pertinent improvement suggestion for key parasitic disease control in the centers for disease control and prevention (CDC) at all levels in China. Methods An on-line questionnaire survey was performed to collect information on the professional staff structure, technique training, laboratory testing capability, and technology needs for parasitic disease control in the CDCs and schistosomioasis control institutions at all levels. The data was analyzed using SPSS 26.0 software. Results A total of 1 855 (provincial, municipal and county) CDCs participated in the survey. Among 122 282 technicians on duty, 7.31% (8 493/122 282) were responsible for parasitic disease control. A total of 4 745 are full-time, accounting for 53.06% (4 745/8 943) of all staff working on parasite disease control. There were significant differences in the distribution of professional titles and educational levels among employees working in CDCs. Higher proportions of senior professional titles and postgraduate degrees were found in the eastern region (19.92%, 13.52%) than in the central (17.02%, 8.29%) and western region (13.88%, 8.25%) (χ² = 1 063.60, 2 892.89;both P < 0.01). The proportion of senior professional titles and postgraduate education among the personnel involved in parasitic disease prevention and control were also higher in the eastern region (21.20%, 12.51%) compared to the central region (15.63%, 7.30%) and western region (11.62%, 6.57%) (χ² = 142.19, 314.93; both P < 0.01). The daily tasks of disease control agency staff at all levels have varying emphases. During the past year, 2 207 technical trainings were given to subordinate CDCs. On average, each individual at provincial CDCs has taken 2.7 training courses, each individual at city-level CDCs has taken 1.4 training courses, and each individual at county-level CDCs has taken 1.1 training courses. There were 12.50% (4/32) of province-level CDCs, 34.02% (83/244) of city-level CDCs and 49.02% (774/1 579) of county-level CDCs without specific laboratories for parasitic diseases. 55.90% (398/712), 40.22% (218/542) and 14.55% (117/804) of CDCs performed the ability for diagnosis of schistosomiasis, echinococcosis and visceral leishmaniasis in provinces where the disease is endemic, and the proportions were higher than those in non-endemic provinces （29.83%, 7.77%, 6.95%）(χ² = 124.36, 283.05, 28.67; all P < 0.01). In addition, the requirements of CDCs mainly focused on personnel training (34.61%, 642/1 855), diagnosing and testing technology (12.40%, 230/1 855), and financial and equipment inputs (5.44%, 101/1 855). Conclusion The CDCs at all levels in the country show capability imbalance in parasitic disease control. Therefore, it is imperative to continuously optimize personnel structure, strengthen professional training and capacity building for laboratory testing, ensuring adequate personnel support for the control and elimination of parasitic diseases.