CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2024 , Vol. 42 >Issue 1: 63 - 68

DOI: https://doi.org/10.12140/j.issn.1000-7423.2024.01.009

ORIGINAL ARTICLES

Infection and molecular characterization of Enterocytozoon bieneusi and Cyclospora cayetanensis in pet dogs and cats in Shanghai

  • QIN Yuan ,
  • LIU Hua ,
  • WANG Yaxue ,
  • ZHANG Jing ,
  • SU Yaxin ,
  • CAO Jianping ,
  • SHEN Yujuan
Expand
  • 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases; Key Laboratory on Parasite and Vector Biology, Ministry of Health; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai 200025, China
    2 School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received date: 2023-09-04

  Revised date: 2023-11-07

  Online published: 2024-03-12

Supported by

Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai (2023—2025) Key Discipline Project(GWⅥ-11.1-09);National Natural Science Foundation of China(82372283);National Science and Technology Major Program of China(2018ZX10713001-004)

Fold

Abstract

Objective To investigate the infection and molecular characteristics of Enterocytozoon bieneusi and Cyclospora cayetanensis in pet dogs and cats in Shanghai and assess the zoonotic potential. Methods Fresh fecal samples were collected from dogs and cats at a pet hospital in Shanghai from November 2021 to June 2022. Genomic DNA was extracted from the fecal samples, and nested PCR was used to amplify the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) of E. bieneusi and the small subunit ribosomal RNA (SSU rRNA) sequence of C. cayetanensis. The positive products were subjected to bidirectional sequencing and sequence alignment by BLAST in the GenBank database. The phylogenetic tree was constructed using MEGA 11.0 software based on the neighbor-joining method. Results A total of 145 fecal samples were collected (99 from dogs and 46 from cats). The positive rates of E. bieneusi and C. cayetanensis were 4.1% (6/145) and 4.8% (7/145), respectively. The positive rates of E. bieneusi and C. cayetanensis in dogs were 3.0% (3/99) and 6.1% (6/99), respectively, while those in cats were 6.5% (3/46) and 2.2% (1/46), respectively. The ITS sequences of 6 E. bieneusi were 100% identical to human genotype A (GenBank accession number: MK982500), and the SSU rRNA sequences of 7 C. cayetanensis were 100% identical to human C. cayetanensis (GenBank accession number: KJ569533). The results of the phylogenetic tree analysis showed that all the E. bieneusi isolates belonged to the same branch as the reported human E. bieneusi genotype A isolates and fell into group 1. All the C. cayetanensis isolates belonged to the same branch as the reported human C. cayetanensis isolates. Conclusion E. bieneusi and C. cayetanensis infections were detected in pet dogs and cats in Shanghai, all of which were caused by zoonotic parasite strains.

Key words： Enterocytozoon bieneusi; Cyclospora cayetanensis; Pets; Zoonotic disease

Cite this article

QIN Yuan , LIU Hua , WANG Yaxue , ZHANG Jing , SU Yaxin , CAO Jianping , SHEN Yujuan . Infection and molecular characterization of Enterocytozoon bieneusi and Cyclospora cayetanensis in pet dogs and cats in Shanghai[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024 , 42(1) : 63 -68 . DOI: 10.12140/j.issn.1000-7423.2024.01.009

References

[1] Xu HL, Jin Y, Wu WX, et al. Genotypes of Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis in dogs and cats in Shanghai, China[J]. Parasit Vectors, 2016, 9: 121.
[2] Shi HS. Prevention measures for sharing parasitic diseases with dogs and cats[J]. Today Anim Husb Vet Med, 2022, 38(1): 30. (in Chinese)
  (石昊书. 人与犬猫共患寄生虫病的防制措施[J]. 今日畜牧兽医, 2022, 38(1): 30.)
[3] Shen YJ, Jiang YY, Cao JP. Prevalence of waterborne intestinal protozoosis and strategies for its prevention and control strategies in China[J]. Chin J Parasitol Parasit Dis, 2021, 39(1): 8-19. (in Chinese)
  (沈玉娟, 姜岩岩, 曹建平. 我国介水传播肠道原虫病流行现状与防控策略[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(1): 8-19.)
[4] Sulaiman IM, Fayer R, Lal AA, et al. Molecular characterization of Microsporidia indicates that wild mammals harbor host-adapted Enterocytozoon spp. as well as human-pathogenic Enterocytozoon bieneusi[J]. Appl Environ Microbiol, 2003, 69(8): 4495-4501.
[5] Li GQ, Xiao SM, Zhou RQ, et al. Molecular characterization of Cyclospora-like organism from dairy cattle[J]. Parasitol Res, 2007, 100(5): 955-961.
[6] Wu Y, Wang JL, Song QQ, et al. Wuhan city pet market status survey[J]. Chin Anim Husb Vet Med, 2007, 34(11): 145-147. (in Chinese)
  (吴岳, 王晶磊, 宋淇淇, 等. 武汉市宠物市场现状调查[J]. 中国畜牧兽医, 2007, 34(11): 145-147.)
[7] Zhang J, Qin Y, Shen YJ, et al. Prevalence and genotyping of Cryptosporidium spp. and Giardia lamblia in dogs and cats from a pet hospital in Shanghai Municipality[J]. Chin J Schisto Contr, 2023, 9(3): 258-262. (in Chinese)
  (张璟, 秦源, 沈玉娟, 等. 上海某宠物医院犬猫隐孢子虫和蓝氏贾第鞭毛虫感染率及基因型鉴定[J]. 中国血吸虫病防治杂志, 2023, 9(3): 258-262.)
[8] Li W, Feng YY, Santin M. Host specificity of Enterocytozoon bieneusi and public health implications[J]. Trends Parasitol, 2019, 35(6): 436-451.
[9] Qi M, Yu FC, Zhao AY, et al. Unusual dominant genotype NIA1 of Enterocytozoon bieneusi in children in Southern Xinjiang, China[J]. PLoS Negl Trop Dis, 2020, 14(6): e0008293.
[10] Li WC, Qin J, Wang K, et al. Genotypes of Enterocytozoon bieneusi in dogs and cats in eastern China[J]. Iran J Parasitol, 2018, 13(3): 457-465.
[11] Karim MR, Dong HJ, Yu FC, et al. Genetic diversity in Enterocytozoon bieneusi isolates from dogs and cats in China: host specificity and public health implications[J]. J Clin Microbiol, 2014, 52(9): 3297-3302.
[12] Wang HY, Lin XH, Sun YX, et al. Occurrence, risk factors and genotypes of Enterocytozoon bieneusi in dogs and cats in Guangzhou, southern China: high genotype diversity and zoonotic concern[J]. BMC Vet Res, 2020, 16(1): 201.
[13] Li W, Li YJ, Song MX, et al. Prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi and Giardia duodenalis in cats and dogs in Heilongjiang Province, China[J]. Vet Parasitol, 2015, 208(3/4): 125-134.
[14] Zhang X, Wang ZX, Su Y, et al. Identification and genotyping of Enterocytozoon bieneusi in China[J]. J Clin Microbiol, 2011, 49(5): 2006-2008.
[15] Zhong YL, Zhou ZY, Deng L, et al. Prevalence and new genotypes of Enterocytozoon bieneusi in sheltered dogs and cats in Sichuan Province, southwestern China[J]. Parasite, 2021, 28: 31.
[16] Wang YG, Zou Y, Yu ZZ, et al. Molecular investigation of zoonotic intestinal Protozoa in pet dogs and cats in Yunnan Province, southwestern China[J]. Pathogens, 2021, 10(9): 1107.
[17] Yai LE, Bauab AR, Hirschfeld MP, et al. The first two cases of Cyclospora in dogs, S?o Paulo, Brazil[J]. Rev Inst Med Trop Sao Paulo, 1997, 39(3): 177-179.
[18] Yue CL, Li GQ, Cheng JL, et al. Development of a specific PCR assay for detecting Cyclospora sp. in dogs[J]. Chin Vet Sci, 2010, 40(11): 1128-1131. (in Chinese)
  (岳彩玲, 李国清, 程家林, 等. 犬源环孢子虫PCR检测方法的建立[J]. 中国兽医科学, 2010, 40(11): 1128-1131.)
[19] Yang LJ. Molecular epidemiology of three diarrhoeal intestinal protozoa in companion animals and laboratory animals in Shanghai[D]. Changchun: Jilin Agricultural University, 2021: 40-50). (in Chinese)
  (杨立娟. 上海地区伴侣动物和实验动物三种致腹泻肠道原虫分子流行病学研究[D]. 长春: 吉林农业大学, 2021: 40-50.)
[20] Totton SC, O’Connor AM, Naganathan T, et al. A review of Cyclospora cayetanensis in animals[J]. Zoonoses Public Health, 2021, 68(8): 861-867.
[21] Ortiz Pineda C, Temesgen TT, Robertson LJ. Multiplex quantitative PCR analysis of strawberries from Bogotá, Colombia, for contamination with three parasites[J]. J Food Prot, 2020, 83(10): 1679-1684.
Options
Outlines

/

〈 〉