CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >
Prevalence and influence factors of Blastocystis infection in residents of Dong ethnic group in Southeast Guizhou Province
Received date: 2023-07-11
Revised date: 2023-09-27
Online published: 2023-12-22
Supported by
Natural Science Foundation of Shanghai(21ZR1469900);National Parasitic Resources Center, and the Ministry of Science and Technology fund(NPRC-2019-194-30);Guizhou Provincial Scientific and Technological Program [(Qian Ke He (2023) General]
Objective To investigate the prevalence and influence factors of Blastocystis in the Dong ethnic group in Guizhou Province in 2022. Methods A cross-sectional survey was conducted through questionnaire investigation in the residents of Mangling Village and Diqing Village in Liping County, Dong ethnic group region, southeast Guizhou Province in 2022. The survey collected general demographic information, hygienic habits, evidence of gastrointestinal symtoms. In addition, fecal samples were collected to extract Blastocystis DNA from which the SSU rRNA gene fragment was amplified by PCR. The positive ampicon was sequenced, and the results were aligned by BLAST in the GenBank database to determine the subgenotype of Blastocystis. The MEGA7.0 software was used to construct phylogenetic tree based on the Blastocystis SSU rRNA gene sequence by neighbor-joining method. Chi-square test and Fisher exact probability method were used to analyze the influencing factors of Blastocystis infection in the residents included in this study. Results A total of 329 village residents were surveyed, including 200 females (60.8%) and 129 males (39.2%), with an average age of 47.95 ± 20.39 years, of which 98.5% (324/329) were of Dong ethnic group, and 1.2% (4/329) were of Han ethnic group. PCR results showed that 16 fecal samples amplified Blastocystis SSU rRNA fragments with a size of about 1 100 bp. The overall Blastocystis infection rate was 4.9% (16/329). There was no significant difference in the infection rates in males (3.1%, 3/129) and females (6.0%, 12/200) (χ2 = 1.425, P > 0.05). The infection rate was higher in the age group over 69 years old, 8.1% (3/37), and there was no significant difference in the infection rate among different age groups (χ2 = 3.865, P > 0.05). The Blastocystis infected individuals were all farmers, and the infection rate was 6.1% (16/261), and there was no significant difference in the infection rate between different occupations (χ2 = 3.157, P > 0.05). The infection rate was the highest in households with 6 or more people (12.7%, 7/55), and the difference in infection rate between different household sizes was statistically significant (Fisher = 13.957, P < 0.01). There were 4 genotypes (ST2, ST3, ST6, ST10) infecting the residents of Mangling Village and Diqing Village. Among them, sub-genotype 6 is the most common, accounting for 50.0% (8/16), followed by sub-genotype 3, accounting for 25.0% (4/16). BLAST comparison analysis showed that the similarity between the positive sequences of 16 Blastocystis and the reference sequences was more than 90%. The phylogenetic tree results also showed that each gene subtype clustered with its reference sequence, and the parentage was high. Univariate analysis showed that drinking water source, family size, daily stool frequency and stool traits have significant differences in Blastocystis infection (χ2 = 8.778, 9.552, 7.384, 15.680; P < 0.05). The results of multivariate analysis showed that drinking unboiled water [OR = 18.347, 95% CI: (3.754, 89.673)] was a risk factor for Blastocystis infection in rural residents. Conclusion The overall infection rate of Blastocystis among Dong ethnic residents in southeast Guizhou Province is comparatively, with the main subgenotype ST6. Drinking unboiled water is the main risk factor for infection with Blastocystis in rural residents.
Key words： Blastocystis sp.; Subtype; Infection rate; Influence factor; Minority
LI Lin , ZHU Aiya , CAI Yuchun , YU Chenghang , CHEN Muxin , TIAN Liguang . Prevalence and influence factors of Blastocystis infection in residents of Dong ethnic group in Southeast Guizhou Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023 , 41(6) : 725 -732 . DOI: 10.12140/j.issn.1000-7423.2023.06.009
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