CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2023 , Vol. 41 >Issue 2: 245 - 248

DOI: https://doi.org/10.12140/j.issn.1000-7423.2023.02.021

SHORT COMMUNICATIONS

Investigation of Blastocystis spp. infection in stray dogs and cats and the parasite genotyping in Zhangzhou, Fujian

  • LI Yongxia ,
  • LI Mengrui ,
  • LI Shiyi ,
  • HUANG Xiaohang ,
  • WU Wei ,
  • HUANG Zhijian ,
  • YIN Guangwen
Expand
  • 1 College of Animal Sciences（College of Bee Science）, Fujian Agriculture and Forestry University，Fuzhou 350002, China
    2 Fujian Animal Drug Engineering Laboratory, Fuzhou 350002, China
    3 Zhangzhou Kangde Pet Hospital, Zhangzhou 363100, Fujian, China
*E-mail: yinguangwen000@sina.com

Received date: 2022-07-05

  Revised date: 2022-10-07

  Online published: 2023-04-23

Supported by

Supported by Cross-sectional Project-Investigation on the Infection of Ticks and Feas in Dogs and Detection of Drug Treatment Effect in Fujian Province(CZ2020107)

Fold

Abstract

To investigate the prevalence of Blastocystis infections among dogs and cats at stray animal shelters in Zhangzhou City, Fujian Province, fecal samples were collected from rescued dogs and cats in Zhangzhou City, and DNA was extracted. Blastocystis ribosomal small subunit RNA (SSU rRNA) was used as a primer for PCR amplification. The positive samples were sent for Sanger sequencing, followed by BLAST sequence alignment analysis using the GenBank public database resources. A phylogenetic tree was constructed using MEGA 7.0 software. A total of 121 cat fecal samples and 119 dog fecal samples were collected. PCR test showed that the Blastocystis positivity rate of 5.04% (6/119) in dogs and 0.82% (1/121) in cats. The difference between dogs and cats was statistically significant (χ2 = 4.153, P < 0.05). Only one fecal sample from a dog was successfully sequenced by PCR amplification of the SSU rRNA gene, yielding the YN-145 sequence. Sequence alignment using BLAST showed a high similarity of 95%-97% with zoonotic Blastocystis ST3 subtype sequences (GenBank accession numbers MW404497.1, MW242639.1, and MW767066.1). Phylogenetic analysis revealed that the YN-145 sequence formed a separate cluster within the ST3 subtype.

Key words： Blastocystis; Dog; Cat; Genotype; Zhangzhou, Fujian

Cite this article

LI Yongxia , LI Mengrui , LI Shiyi , HUANG Xiaohang , WU Wei , HUANG Zhijian , YIN Guangwen . Investigation of Blastocystis spp. infection in stray dogs and cats and the parasite genotyping in Zhangzhou, Fujian[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023 , 41(2) : 245 -248 . DOI: 10.12140/j.issn.1000-7423.2023.02.021

References

[1] Stensvold CR, Clark CG. Current status of Blastocystis: a personal view[J]. ParasitolInt, 2016, 65(6 Pt B): 763-771.
[2] Liu LK, Wang PL, Han H, et al. Current status of Blastocystis infection in China[J]. Chin J Zoonoses, 2021, 37(6): 548-555, 562. (in Chinese)
  (刘林科, 王朋林, 韩涵, 等. 我国芽囊原虫感染现状[J]. 中国人兽共患病学报, 2021, 37(6): 548-555, 562.)
[3] Sun Q, Yan YM. Research progress of the pathogenesis of Blastocystis[J]. Chin J Zoonoses, 2019, 35(4): 345-349, 354. (in Chinese)
  (孙沁, 严宜明. 芽囊原虫致病机制的研究进展[J]. 中国人兽共患病学报, 2019, 35(4): 345-349, 354.)
[4] Chen HH, Li YX, Ning CQ, et al. Current prevalence and sub-genotype analysis of Blastocystis in residents of Luoyang City[J]. Chin J Parasitol Parasit Dis, 2022, 40(2): 216-222. (in Chinese)
  (陈慧慧, 李云霞, 宁超群, 等. 洛阳市人群芽囊原虫感染现状及基因亚型分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(2): 216-222.)
[5] Pu LH, Zhang XZ, Guan SJ, et al. Blastocystis sp. infection in dairy cattle in Yunnan Province and its gene subtype analysis[J]. Chin J Parasitol Parasit Dis, 2021, 39(6): 809-815. (in Chinese)
  (普丽花, 张兴泽, 关少君, 等. 云南省奶牛芽囊原虫的感染情况及其基因亚型分析[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(6): 809-815.)
[6] Liu LK, Wang PL, Jian YC, et al. Epidemiological investigation of Blastocystis from sheep and goats in some areas of China[J]. Acta Vet Zootechnica Sin, 2021, 52(4): 1061-1068. (in Chinese)
  (刘林科, 王朋林, 菅忆晨, 等. 我国部分地区羊芽囊原虫流行病学调查[J]. 畜牧兽医学报, 2021, 52(4): 1061-1068.)
[7] Chen LY, Chai YJ, Yao JX, et al. Investigation of infection and genotyping of Blastocystis sp. in a wildlife park in Guiyang[J]. J Yunnan Agric Univ Nat Sci, 2021, 36(5): 925-929. (in Chinese)
  (陈礼颖, 柴宜均, 姚静新, 等. 贵阳某野生动物园芽囊原虫感染调查及基因分型[J]. 云南农业大学学报(自然科学), 2021, 36(5): 925-929.)
[8] Scanlan PD, Stensvold CR, Rajili?-Stojanovi? M, et al. The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota[J]. FEMS Microbiol Ecol, 2014, 90(1): 326-330.
[9] Petersen AM, Stensvold CR, Mirsepasi H, et al. Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection[J]. Scand J Gastroenterol, 2013, 48(5): 638-639.
[10] Scicluna SM, Tawari B, Clark CG. DNA barcoding of blastocystis[J]. Protist, 2006, 157(1): 77-85.
[11] Kaczmarek A, Rocka A, Weso?owska M, et al.. Blastocystis isolates from a dog and their owners presenting with chronic diarrhoea Dogs as reservoirs of Blastocystis: research in Poland and worldwide[J]. Ann Parasitol, 2020, 66(4): 573-579.
[12] Gazzonis AL, Marangi M, Zanzani SA, et al. Molecular epidemiology of Blastocystis sp. in dogs housed in Italian rescue shelters[J]. Parasitol Res, 2019, 118(10): 3011-3017.
[13] Belleza MLB, Cadacio JLC, Borja MP, et al. Epidemiologic study of Blastocystis infection in an urban community in the Philippines[J]. J Environ Public Health, 2015, 2015: 894297.
[14] Chen Y, He GF, Xia YX, et al. Epidemiological investigation on Blastocystis in pet dogs from some cities in Henan Province[J]. Chin J Vet Med, 2021, 57(7): 103-107. (in Chinese)
  (陈益, 贺桂芬, 夏艳勋, 等. 河南省部分城市宠物犬芽囊原虫的感染情况调查[J]. 中国兽医杂志, 2021, 57(7): 103-107.)
[15] Li WC, Wang K, Qin M, et al. Blastocystis infection in pet dogs in selected areas of Anhui and Zhejiang Provinces[J]. Chin J Parasitol Parasit Dis, 2016, 34(3): 272-276. (in Chinese)
  (李文超, 汪凯, 秦勉, 等. 皖浙两省部分地区宠物犬芽囊原虫的感染情况[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(3): 272-276.)
[16] Wang JG, Gong BY, Liu XH, et al. Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China[J]. Parasit Vectors, 2018, 11(1): 522.
[17] Liao SQ, Lin XH, Sun YX, et al. Occurrence and genotypes of Cryptosporidium spp., Giardia duodenalis, and Blastocystis sp. in household, shelter, breeding, and pet market dogs in Guangzhou, Southern China[J]. Sci Rep, 2020, 10(1): 17736.
[18] Li WC, Liu XC, Gu YF, et al. Prevalence of Cryptosporidium, Giardia, Blastocystis, and trichomonads in domestic cats in East China[J]. J Vet Med Sci, 2019, 81(6): 890-896.
Options
Outlines

/

〈 〉