CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2022 , Vol. 40 >Issue 5: 677 - 681

DOI: https://doi.org/10.12140/j.issn.1000-7423.2022.05.018

SHORT COMMUNICATIONS

Complete mitochondrial genome sequence of Rhipicephalus microplus

  • LIU Ya-fang ,
  • CHEN Bin ,
  • LU Xin-yan ,
  • LI Guang-hua ,
  • DU Chun-hong ,
  • JIANG Dan-dan ,
  • YANG Xing
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  • 1. School of Basic Medicine, Dali University, Dali 671000, China
    2. Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China
    3. School of Public Health, Dali University, Dali 671000, China

Received date: 2022-01-24

  Revised date: 2022-04-09

  Online published: 2022-08-02

Supported by

National Natural Science Foundation of China(81760607);National Natural Science Foundation of China(U2002219);Yunnan Natural Science Foundation(2017FD139);Scientific Research Fund of Yunnan Education Department(2022J0687)

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Abstract

Rhipicephalus microplus was collected by the cloth flag method in Yunnan Province in September 2017 and was used for DNA extraction. Four long fragments were amplified by long PCR using the primers designed based on 16S rRNA, 12S rRNA, nad1 and cox3 gene sequences. The fragments were sequenced by conserved primer-walking. The complete mitochondrial genome sequence was obtained by splicing. The length of the mitochondrial genome (mtDNA) of Rhipicephalus microplus is 14 763 bp. It contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a non-coding control region of 264 bp (D-Loop region). The base composition was A 38.66%, G 9.02%, C 11.46%, T 40.87%. A + T content was 79.53%. The starting codon of protein-coding genes was ATN. The stop codons were TAA or TAG, except for cox2, cox3, nad5, which used incomplete T as the stop codon. The complete mitochondrial genome have 12 gene overlaps. The length ranges from 1-24 bp. The length of 18 total intergenic regions was 433 bp ranging from 1-311 bp. A total of 22 transferring RNA were found, all of which were typical cloverleaf structures except for tRNACys, tRNAPhe, tRNASer.

Key words： Rhipicephalus microplus; Mitochondrial genome; Sequence analysis

Cite this article

LIU Ya-fang , CHEN Bin , LU Xin-yan , LI Guang-hua , DU Chun-hong , JIANG Dan-dan , YANG Xing . Complete mitochondrial genome sequence of Rhipicephalus microplus[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022 , 40(5) : 677 -681 . DOI: 10.12140/j.issn.1000-7423.2022.05.018

References

[1] Burnard D,, Shao RF. Mitochondrial genome analysis reveals intraspecific variation within Australian hard tick species[J]. Ticks Tick Borne Dis, 2019, 10(3): 677-681.
[2] Wang TH,, Zhang SQ,, Pei TW, et al. Tick mitochondrial genomes: structural characteristics and phylogenetic implications[J]. Parasit Vectors, 2019, 12(1): 451.
[3] Esser HJ,, Herre EA,, Blüthgen N, et al. Host specificity in a diverse Neotropical tick community: an assessment using quantitative network analysis and host phylogeny[J]. Parasit Vectors, 2016, 9(1): 372.
[4] Burger TD,, Shao RF,, Barker SC. Phylogenetic analysis of mitochondrial genome sequences indicates that the cattle tick, Rhipicephalus (Boophilus) microplus, contains a cryptic species[J]. Mol Phylogenet Evol, 2014, 76: 241-253.
[5] Zhuang L,, Sun Y,, Cui XM, et al. Transmission of severe fever with thrombocytopenia syndrome virus by Haemaphysalis longicornis ticks, China[J]. Emerg Infect Dis, 2018, 24(5): 868-871.
[6] Beati L,, Keirans JE. Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari ∶ Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters[J]. J Parasitol, 2001, 87(1): 32-48.
[7] Li ZB,, Yao GM,, Luo W, et al. Genetic variation and phylogenetic evolution of Haemaphysalis flava from different geographical areas in China[J]. China Animal Husb & Vet Med, 2021, 48(6): 2150-2159. (in Chinese)
[7] ( 李中波,, 尧国民,, 罗维, 等. 不同地区褐黄血蜱的基因变异及遗传进化分析[J]. 中国畜牧兽医, 2021, 48(6): 2150-2159.)
[8] Simon C,, Buckley TR,, Frati F, et al. Incorporating molecular evolution into phylogenetic analysis, and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA[J]. Annu Rev Ecol Evol Syst, 2006, 37: 545-579.
[9] Sun ET,, Li CP,, Nie LW, et al. The complete mitochondrial genome of the brown leg mite, Aleuroglyphus ovatus (Acari ∶ Sarcoptiformes): evaluation of largest non-coding region and unique tRNAs[J]. Exp Appl Acarol, 2014, 64(2): 141-157.
[10] Yuan ML,, Wei DD,, Zhang K, et al. Genetic diversity and population structure of Panonychus citri (Acari ∶ Tetranychidae), in China based on mitochondrial COⅠ gene sequences[J]. J Econ Entomol, 2010, 103(6): 2204-2213.
[11] De AK,, Muthiyan R,, Ponraj P, et al. Mitogenome analysis of Indian isolate of Rhipicephalus microplus clade A sensu (Burger et al.,2014): a first report from Maritime South-East Asia[J]. Mitochondrion, 2019, 49: 135-148.
[12] Ji XF,, Wu XB,, Li Y, et al. The mitochondrial genome of Crocodylus niloticus with implications for phylogenetic relationships among crocodilian species[J]. Acta Zool Sin, 2006, 52(4): 810-818. (in Chinese)
[12] ( 季学峰,, 吴孝兵,, 李艳, 等. 尼罗鳄线粒体基因组全序列分析及鳄类系统发生关系的探讨[J]. 动物学报, 2006, 52(4): 810-818.)
[13] Liang S,, Hu SS,, Zhou J, et al. Sequencing and analysis of mitochondrial genome in new Zealand white rabbit[J]. Genom Appl Biol, 2021, 40(1): 28-33. (in Chinese)
[13] ( 梁爽,, 胡帅帅,, 周娟, 等. 新西兰白兔线粒体基因组全序列的测定与分析[J]. 基因组学与应用生物学, 2021, 40(1): 28-33.)
[14] Xie AX,, Wu QC,, Li SD, et al. Complete sequence cloning and bioinformatics analysis of Sichuan sheldrake duck mitochondrial genome[J]. Chin J Animal Vet Sci, 2017, 48(3): 436-445. (in Chinese)
[14] ( 谢艾轩,, 吴启超,, 李思德, 等. 四川麻鸭mtDNA全序列的克隆和生物信息学分析[J]. 畜牧兽医学报, 2017, 48(3): 436-445.)
[15] Zhang DX,, Hewitt GM. Insect mitochondrial control region: a review of its structure, evolution and usefulness in evolutionary studies[J]. Biochem Syst Ecol, 1997, 25(2): 99-120.
[16] Wolstenholme DR. Animal mitochondrial DNA: structure and evolution[J]. Int Rev Cytol, 1992, 141: 173-216.
[17] Arndt A,, Smith MJ. Mitochondrial gene rearrangement in the sea cucumber genus Cucumaria[J]. Mol Biol Evol, 1998, 15(8): 1009-1016.
[18] Umeda S,, Tang Y,, Okamoto M, et al. Both heavy strand replication origins are active in partially duplicated human mitochondrial DNAs[J]. Biochem Biophys Res Commun, 2001, 286(4): 681-687.
[19] Shao R,, Aoki Y,, Mitani H, et al. The mitochondrial genomes of soft ticks have an arrangement of genes that has remained unchanged for over 400 million years[J]. Insect Mol Biol, 2004, 13(3): 219-224.
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