CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2022 , Vol. 40 >Issue 5: 610 - 615

DOI: https://doi.org/10.12140/j.issn.1000-7423.2022.05.007

ORIGINAL ARTICLES

Genetic diversity and differentiation time of human isolates of Echinococcus granulosus and E. multilocularis from Qinghai

  • WU De-fang ,
  • FU Yong ,
  • REN Bin ,
  • ZHANG Yao-gang ,
  • XU Xiao-lei ,
  • PANG Ming-quan ,
  • FAN Hai-ning
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  • 1. Affiliated Hospital of Qinghai University, Qinghai Research Key Laboratory for Echinococcosis, Xining 810001, China
    2. Qinghai Academy of Animal Science and Veterinary Medicine, Xining 810001, China

Received date: 2021-12-30

  Revised date: 2022-02-26

  Online published: 2022-10-24

Supported by

National Natural Science Foundation of China(81960576);Project of Science and Technology Department of Qinghai Province(2021-ZJ-724)

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Abstract

Objective To analyzed the genetic diversity, genetic differences between populations and differentiation time of Echinococcus granulosus and E. multilocularis of Qinghai isolates, in order to provide scientific basis for species tracing and prevention and control of Echinococcus in Qinghai Province, China. Methods For genetic analysis, 50 liver lesion samples were collected from hospitalized echinococcosis patients in the Affiliated Hospital of Qinghai University to extract genomic DNA and amplify mitochondrial dehydrogenase 1 gene (nad1). Sequence multiple alignment was performed using Clustal X v2.0 software. Geographic informatics mapping of patients’ residence was constructed using ArcGIS software. Sequence haplotype analysis was made with DnaSP v6 software. Modeltest 3.7 software and PAUP*4.0B10 software were used to calculate the minimum optimal nucleic acid evolution model. The Bayesian’s phylogenetic evolution tree was constructed with MrBayes-3.2.7 software. The differentiation time of each node in the phylogenetic tree was estimated with the Bayesian method using BEAST v2.6.3 software. Results We successfully identified 48 Echinococcus lesion samples specimen and obtained the full length of complete nad1 gene of 894 bp. Among them, 13 samples were identified as the G1 genotype of E. granulosus, and 35 samples as E. multilocularis. All the sequences showed > 99% similarity to those in GenBank. Four haplotypes were identified as H1-H4 in the two species respectively; H3 was the dominant haplotype in E. granulosus samples(10/13), which is present in Xining, Guoluo, Yushu, Haidong, Haibei and Huangnan. H2 haplotype was found dominant in E. multilocular samples (51.4%,18/35), which is present in Xining, Guoluo, Yushu, and Haidong. The phylogenetic tree showed that E. granulosus and G1 genotype clustered into one branch, and E. multilocularis and Asian strain clustered into one branch. The results of differentiation time showed that the nearest common ancestor of E. granulosus, E. multilocularis, E. vogeli and E. oligarthrus was about 5.5 Mya (95% confidence interval 4.5-6.5 Mya), and the differentiation time of E. granulosus and E. multilocularis was about 2.5 Mya (95% confidence interval 2.3-4.1 Mya). Conclusion Both human E. granulosus and E. multilocularis in Qinghai Province show high genetic diversity. E. granulosus was found of G1 genotype, with H3 as the dominant haplotype, while in E. multilocularis samles H2 is the dominant. The two speies are widely distributed throughout Qinghai Province. The two species of Echinococcus exhit closer genetic relationship and differentiation timing.

Key words： Echinococcus granulosus; Echinococcus multilocularis; nad1 gene; Haplotype; Differentiation time

Cite this article

WU De-fang , FU Yong , REN Bin , ZHANG Yao-gang , XU Xiao-lei , PANG Ming-quan , FAN Hai-ning . Genetic diversity and differentiation time of human isolates of Echinococcus granulosus and E. multilocularis from Qinghai[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022 , 40(5) : 610 -615 . DOI: 10.12140/j.issn.1000-7423.2022.05.007

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