CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >
Blastocystis infection and its effect on blood parameters in laboratory mice
Received date: 2021-08-24
Revised date: 2021-11-15
Online published: 2022-04-15
Supported by
S&T Program of Hebei(H2021205002)
To analyze the infection of Blastocystis and its effect on blood parameters in laboratory mice. Fecal samples of clean-grade BALB/c mice raised in 6 different laboratories in Shijiazhuang City were collected from 2020 to 2021. The fecal DNA was extracted, and then the 18S-specific fragments of Blastocystis were amplified, sequenced and BLAST in the GenBank database. The subtypes of Blastocystis were analyzed, and the phylogenetic tree based on the 18S sequence was constructed by the maximum likelihood method. Five mice infected with Blastocystis and five uninfected mice raised in the same conditions were selected to collect blood samples for the routine blood test. The results were analyzed by t-test to compare the differences between the two groups. A total of 238 fecal samples were collected, and 47 were Blastocystis positive with an infection rate of 19.8% (47/238). The sequence of the 47 positive samples displayed 80.31%-82.79%, 97.84%-99.13% and 98.79% identity to MK782501.1 (ST1), MN338076.1 (ST2) and MK861934.1 (ST7), respectively. The phylogenetic tree showed that the sequences of positive samples were clustered into a branch with the sequences of ST1, ST2 and ST7 subtypes, respectively. The results of the blood test showed that the average hemoglobin content in mice infected with Blastocystis was (21.17 ± 0.45) pg, which was lower than that in the uninfected mice (23.90 ± 0.60) pg(t = 6.308, P < 0.01). The average hemoglobin concentration was (480.00 ± 23.64) g/L, which was lower than that in the uninfected mice (544.33 ± 15.70) g/L(t = 3.927, P < 0.05), while there was no significant difference in other indicators (P > 0.05). The results of this study suggest that the subtypes of Blastocystis which the mice infected were ST1, ST2 and ST7. The infection of Blastocystis will result in abnormal blood parameters in the mice.
Key words： Laboratory mouse; Blastocystis sp.; Subtype; Blood routine test
QIAO Hai-xia , CAO Meng-juan , HUANG Jing , MA Lei . Blastocystis infection and its effect on blood parameters in laboratory mice[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022 , 40(2) : 252 -255 . DOI: 10.12140/j.issn.1000-7423.2022.02.019
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